Smad protein and TGF-β signaling in vascular smooth muscle cells

  • Authors:
    • Hirofumi Ikedo
    • Kiyoshi Tamaki
    • Seiji Ueda
    • Seiya Kato
    • Makiko Fujii
    • Peter ten Dijke
    • Seiya Okuda
  • View Affiliations

  • Published online on: May 1, 2003     https://doi.org/10.3892/ijmm.11.5.645
  • Pages: 645-650
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Abstract

Transforming growth factor-β1 (TGF-β1) plays a role in vascular remodeling by stimulating vascular smooth muscle cell (SMC) growth and matrix-protein synthesis at sites of vascular injury. Smad proteins have been shown to mediate intracellular signaling of this growth factor. We investigated the expression and phosphorylation of Smads in cultured rat aortic smooth muscle cells. In addition, we evaluated the effects of overexpression of Smad proteins on TGF-β signal transduction by adenovirus-mediated gene transfer. In rat SMC, Smad1, Smad2, Smad3, Smad4 and Smad5 were detected by immunoprecipitation. Using antisera against phosphorylated Smad2, we showed that TGF-β1-induced Smad2 phosphorylation in a concentration- and time-dependent manner. Using adenovirus-mediated transfection method, we demonstrated that overexpression of Smad2 or Smad4 was associated with an increased production of TGF-β1-induced plasminogen activator inhibitor-1 (PAI-1). However, the most prominent expression of PAI-1 was observed upon cotransfection of both Smad2 and Smad4. Both the proliferative effect of TGF-β1 under serum-free conditions and its anti-proliferative effect under serum-rich conditions were suppressed by the adenovirus-mediated overexpression of Smad7. These results indicated that Smads proteins were expressed in vascular SMC and that they mediated TGF-β signaling in those cells.

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May 2003
Volume 11 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Ikedo H, Tamaki K, Ueda S, Kato S, Fujii M, ten Dijke P and Okuda S: Smad protein and TGF-β signaling in vascular smooth muscle cells. Int J Mol Med 11: 645-650, 2003
APA
Ikedo, H., Tamaki, K., Ueda, S., Kato, S., Fujii, M., ten Dijke, P., & Okuda, S. (2003). Smad protein and TGF-β signaling in vascular smooth muscle cells. International Journal of Molecular Medicine, 11, 645-650. https://doi.org/10.3892/ijmm.11.5.645
MLA
Ikedo, H., Tamaki, K., Ueda, S., Kato, S., Fujii, M., ten Dijke, P., Okuda, S."Smad protein and TGF-β signaling in vascular smooth muscle cells". International Journal of Molecular Medicine 11.5 (2003): 645-650.
Chicago
Ikedo, H., Tamaki, K., Ueda, S., Kato, S., Fujii, M., ten Dijke, P., Okuda, S."Smad protein and TGF-β signaling in vascular smooth muscle cells". International Journal of Molecular Medicine 11, no. 5 (2003): 645-650. https://doi.org/10.3892/ijmm.11.5.645