IL-10 in vivo gene expression in a cell-induced animal model of proliferative vitreoretinopathy

  • Authors:
    • Ernesto Bali
    • François Willermain
    • Laure Caspers-Velu
    • Christine Dubois
    • Marie-Françoise Dehou
    • Thierry Velu
    • Jacques Libert
    • Catherine Bruyns
  • View Affiliations

  • Published online on: September 1, 2003     https://doi.org/10.3892/ijmm.12.3.305
  • Pages: 305-310
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Abstract

Due to inhibitory activities on cell-mediated immune responses, interleukin-10 (IL-10) has been proposed as a good candidate to treat inflammatory eye disease and proliferative vitreoretinopathy (PVR). In this study we evaluate the effect of human IL-10 (hIL-10) expression in a cell-induced animal model of PVR. Rabbit dermal fibroblasts were genetically modified by infection with retroviral particles carrying the neomycin resistance gene (neoR) alone or in combination with the hIL-10 gene. PVR was induced in rabbits by intravitreal injections of RDF hIL-10 or RDF neo. Some rabbits received instead injections of soluble recombinant hIL-10 (rhIL-10). PVR was graded by fundoscopy. Eyes were enucleated for histology at day 28. ELISA was performed to measure hIL-10 production in RDF supernatants and in vitreous samples, 24 h after injection. Results showed that in vitro hIL-10 production by RDF was 24,500 pg/106 cells/ml/24 h. In vivo IL-10 secretion was detected in all rabbits injected with RDF hIL-10 but was undetectable in control rabbits. Similar clinical grades of PVR were found in rabbits injected with RDF hIL-10 or RDF neo. Histology showed that all eyes injected with RDF hIL-10 had significant inflammatory infiltration whereas only one control eye was clearly inflamed. Rabbits injected with soluble rhIL-10 had normal fundoscopy and normal histology. In conclusion, our results show that in vivo, in a cell-induced model of PVR, hIL-10 has no effect in the clinical progression of PVR. Histology, however, shows that pro-inflammatory effects seem to overcome its suppressive properties.

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September 2003
Volume 12 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Bali E, Willermain F, Caspers-Velu L, Dubois C, Dehou M, Velu T, Libert J and Bruyns C: IL-10 in vivo gene expression in a cell-induced animal model of proliferative vitreoretinopathy. Int J Mol Med 12: 305-310, 2003
APA
Bali, E., Willermain, F., Caspers-Velu, L., Dubois, C., Dehou, M., Velu, T. ... Bruyns, C. (2003). IL-10 in vivo gene expression in a cell-induced animal model of proliferative vitreoretinopathy. International Journal of Molecular Medicine, 12, 305-310. https://doi.org/10.3892/ijmm.12.3.305
MLA
Bali, E., Willermain, F., Caspers-Velu, L., Dubois, C., Dehou, M., Velu, T., Libert, J., Bruyns, C."IL-10 in vivo gene expression in a cell-induced animal model of proliferative vitreoretinopathy". International Journal of Molecular Medicine 12.3 (2003): 305-310.
Chicago
Bali, E., Willermain, F., Caspers-Velu, L., Dubois, C., Dehou, M., Velu, T., Libert, J., Bruyns, C."IL-10 in vivo gene expression in a cell-induced animal model of proliferative vitreoretinopathy". International Journal of Molecular Medicine 12, no. 3 (2003): 305-310. https://doi.org/10.3892/ijmm.12.3.305