Inhibition of the O-glycan elongation limits MUC1 incorporation to cell membrane of human endometrial carcinoma cells

  • Authors:
    • Halina Porowska
    • Anna Paszkiewicz-Gadek
    • Tomasz Anchim
    • Slawomir Wolczynski
    • Andrzej Gindzienski
  • View Affiliations

  • Published online on: March 1, 2004     https://doi.org/10.3892/ijmm.13.3.459
  • Pages: 459-464
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

We have studied how benzyl-N-acetyl-α-D-galactosaminide, O-glycosylation inhibitor, affects the polymorphism and shedding of membrane-bound MUC1 mucin, and change in adhesive properties of cancer cells. In endometrial adenocarcinoma cells (Ishikawa line), high molecular weight MUC1 mucin was shed from cellular membrane and could be detected in culture medium 24 h after [14C]threonine labelling. Short-time (2 days) exposure of these cells to benzyl-N-acetyl-α-D-galactosaminide was associated with a reduction in sialic acid level and increase in T antigen content in cellular MUC1 mucin. These changes could be inverted after removal of the inhibitor. A longer, 6-day action of the inhibitor induced a decrease in sialic acid and T antigen levels in cellular MUC1 mucin. Benzyl-N-acetyl-α-D-galactosaminide treatment caused the occurrence of a few incompletely glycosylated glycoforms of MUC1 in cells, but not in culture medium. Adhesion of endometrial cells to ECM compounds (type I collagen) was increased by benzyl-N-acetyl-α-D-galactosaminide treatment, indicating that glycosylation of extracellular domain of MUC1 can modulate adhesive properties of cells.

Related Articles

Journal Cover

March 2004
Volume 13 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Porowska H, Paszkiewicz-Gadek A, Anchim T, Wolczynski S and Gindzienski A: Inhibition of the O-glycan elongation limits MUC1 incorporation to cell membrane of human endometrial carcinoma cells. Int J Mol Med 13: 459-464, 2004
APA
Porowska, H., Paszkiewicz-Gadek, A., Anchim, T., Wolczynski, S., & Gindzienski, A. (2004). Inhibition of the O-glycan elongation limits MUC1 incorporation to cell membrane of human endometrial carcinoma cells. International Journal of Molecular Medicine, 13, 459-464. https://doi.org/10.3892/ijmm.13.3.459
MLA
Porowska, H., Paszkiewicz-Gadek, A., Anchim, T., Wolczynski, S., Gindzienski, A."Inhibition of the O-glycan elongation limits MUC1 incorporation to cell membrane of human endometrial carcinoma cells". International Journal of Molecular Medicine 13.3 (2004): 459-464.
Chicago
Porowska, H., Paszkiewicz-Gadek, A., Anchim, T., Wolczynski, S., Gindzienski, A."Inhibition of the O-glycan elongation limits MUC1 incorporation to cell membrane of human endometrial carcinoma cells". International Journal of Molecular Medicine 13, no. 3 (2004): 459-464. https://doi.org/10.3892/ijmm.13.3.459