Early effects of denervation on Ca2+-handling proteins in skeletal muscle

  • Authors:
    • Pamela Donoghue
    • Samo Ribaric
    • Brendan Moran
    • Vita Cebasek
    • Ida Erzen
    • Kay Ohlendieck
  • View Affiliations

  • Published online on: June 1, 2004     https://doi.org/10.3892/ijmm.13.6.767
  • Pages: 767-772
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Abstract

The adaptive response of skeletal muscle fibres depends on a variety of biological factors including loading conditions and neuromuscular activity. An extreme type of atrophy-inducing change in contractile activity is represented by the physical disconnection between the motor nerve and its respective fibre unit. Since fibre type alterations have a striking effect on the Ca2+-regulatory apparatus, we have investigated the fate of a key Ca2+-pump and essential Ca2+-binding proteins in extensor digitorum longus specimens two weeks after nerve crush or complete denervation. In contrast to increased levels of sarcalumenin, immunoblotting revealed that the expression of the fast SERCA1 Ca2+-ATPase isoform is drastically decreased and fast calsequestrin is slightly reduced. Analysis of myosin heavy chain isoforms agreed with this result and showed a fast-to-slow fibre type shifting process following denervation. Hence, changes in muscle activity appear to have a profound effect on the abundance and isoform expression pattern of Ca2+-handling elements.

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June 2004
Volume 13 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Donoghue P, Ribaric S, Moran B, Cebasek V, Erzen I and Ohlendieck K: Early effects of denervation on Ca2+-handling proteins in skeletal muscle. Int J Mol Med 13: 767-772, 2004
APA
Donoghue, P., Ribaric, S., Moran, B., Cebasek, V., Erzen, I., & Ohlendieck, K. (2004). Early effects of denervation on Ca2+-handling proteins in skeletal muscle. International Journal of Molecular Medicine, 13, 767-772. https://doi.org/10.3892/ijmm.13.6.767
MLA
Donoghue, P., Ribaric, S., Moran, B., Cebasek, V., Erzen, I., Ohlendieck, K."Early effects of denervation on Ca2+-handling proteins in skeletal muscle". International Journal of Molecular Medicine 13.6 (2004): 767-772.
Chicago
Donoghue, P., Ribaric, S., Moran, B., Cebasek, V., Erzen, I., Ohlendieck, K."Early effects of denervation on Ca2+-handling proteins in skeletal muscle". International Journal of Molecular Medicine 13, no. 6 (2004): 767-772. https://doi.org/10.3892/ijmm.13.6.767