Delayed interconversion of D-glucose anomers in D2O

  • Authors:
    • Willy J. Malaisse
    • Ingrid Verbruggen
    • Monique Biesemans
    • Rudolph Willem
  • View Affiliations

  • Published online on: June 1, 2004     https://doi.org/10.3892/ijmm.13.6.855
  • Pages: 855-857
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The anomeric specificity of D-glucose metabolism and functional effects is currently assessed either over about 5 min at 37°C or longer periods at much lower temperatures. In the present study, the half-life for the interconversion of D-glucose anomers at 30°C was found to be increased (P<0.005) from 17.2±0.9 min in a mixture of H2O/D2O (9/1, v/v) to 58.6±1.9 min in pure D2O. It is proposed that advantage can be taken from this delayed interconversion in D2O in experiments dealing with the anomeric specificity of D-glucose metabolic fate and functional effects. Thus, even over 60-min incubation at 30°C, the integrated mean abundance of α- and β-D-glucose would remain close to 82 and 89%, respectively, of the corresponding initial value (100%).

Related Articles

Journal Cover

June 2004
Volume 13 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Malaisse WJ, Verbruggen I, Biesemans M and Willem R: Delayed interconversion of D-glucose anomers in D2O. Int J Mol Med 13: 855-857, 2004
APA
Malaisse, W.J., Verbruggen, I., Biesemans, M., & Willem, R. (2004). Delayed interconversion of D-glucose anomers in D2O. International Journal of Molecular Medicine, 13, 855-857. https://doi.org/10.3892/ijmm.13.6.855
MLA
Malaisse, W. J., Verbruggen, I., Biesemans, M., Willem, R."Delayed interconversion of D-glucose anomers in D2O". International Journal of Molecular Medicine 13.6 (2004): 855-857.
Chicago
Malaisse, W. J., Verbruggen, I., Biesemans, M., Willem, R."Delayed interconversion of D-glucose anomers in D2O". International Journal of Molecular Medicine 13, no. 6 (2004): 855-857. https://doi.org/10.3892/ijmm.13.6.855