Cross talk between COX-2 inhibitor and hyaluronic acid in osteoarthritic chondrocytes

  • Authors:
    • Toshiaki Takahashi
    • Yoshiki Uemura
    • Hirokuni Taguchi
    • Yasuhiro Ogawa
    • Shoji Yoshida
    • Michio Toda
    • Toshihiro Kobayashi
    • Harumichi Seguchi
    • Toshikazu Tani
  • View Affiliations

  • Published online on: August 1, 2004     https://doi.org/10.3892/ijmm.14.2.139
  • Pages: 139-144
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Abstract

Both hyaluronic acid (HA) and cyclooxygenase-2 (COX-2) inhibitors are used in clinical practice in the treatment of osteoarthritis. There have been no reports regarding cross-talk between HA and COX-2 inhibitors in articular human chondrocytes. The purpose of this study was to investigate whether HA, COX-2 inhibitors or a combination of COX-2 inhibitors and HA have different effects in human articular between lower and highly degenerated chondrocytes. Isolated lower and highly degenerated chondrocytes were divided into 5 groups: ethanol (used as a control for the solvents), HA, COX-2 inhibitors, COX-2 inhibitors plus HA, or no additive. After incubating for 48 h, mitochondrial membrane potential analysis and western blotting of p38 and p44/42 mitogen-activated protein kinase (MAPK) were performed. Glycosaminoglycan, nitric oxide (NO) production and prostaglandin E2 (PGE2) concentrations were assessed. A combination of COX-2 inhibitors and HA resulted in dendritic, proliferating chondrocytes with strong red fluorescence enriched in the mitochondrial membrane, and indicated reduction of apoptosis in chondrocytes. COX-2 inhibitors alone, and a combination of COX-2 inhibitor and HA inhibited the activation of p38 in highly degenerated chondrocytes. A combination of COX-2 inhibitors and HA decreased NO production in highly degenerated chondrocytes. COX-2 inhibitors decreased PGE2 production, however, HA alone had no effect on PGE2 production. The present study demonstrated that COX-2 inhibitors and HA interacted synergistically the MAPK pathway and inhibition of NO production in highly degenerated chondrocytes. Administration of COX-2 inhibitors plus HA could be used as a new alternative way of treating osteoarthritis.

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August 2004
Volume 14 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Takahashi T, Uemura Y, Taguchi H, Ogawa Y, Yoshida S, Toda M, Kobayashi T, Seguchi H and Tani T: Cross talk between COX-2 inhibitor and hyaluronic acid in osteoarthritic chondrocytes. Int J Mol Med 14: 139-144, 2004
APA
Takahashi, T., Uemura, Y., Taguchi, H., Ogawa, Y., Yoshida, S., Toda, M. ... Tani, T. (2004). Cross talk between COX-2 inhibitor and hyaluronic acid in osteoarthritic chondrocytes. International Journal of Molecular Medicine, 14, 139-144. https://doi.org/10.3892/ijmm.14.2.139
MLA
Takahashi, T., Uemura, Y., Taguchi, H., Ogawa, Y., Yoshida, S., Toda, M., Kobayashi, T., Seguchi, H., Tani, T."Cross talk between COX-2 inhibitor and hyaluronic acid in osteoarthritic chondrocytes". International Journal of Molecular Medicine 14.2 (2004): 139-144.
Chicago
Takahashi, T., Uemura, Y., Taguchi, H., Ogawa, Y., Yoshida, S., Toda, M., Kobayashi, T., Seguchi, H., Tani, T."Cross talk between COX-2 inhibitor and hyaluronic acid in osteoarthritic chondrocytes". International Journal of Molecular Medicine 14, no. 2 (2004): 139-144. https://doi.org/10.3892/ijmm.14.2.139