Induction of apoptotic cell death by mycelium extracts of Phellinus linteus in human neuroblastoma cells

  • Authors:
    • Yung Hyun Choi
    • Man Kyu Huh
    • Chung-Ho Ryu
    • Byung Tae Choi
    • Young-Kee Jeong
  • View Affiliations

  • Published online on: August 1, 2004     https://doi.org/10.3892/ijmm.14.2.227
  • Pages: 227-232
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Phellinus linteus is a well-known Oriental medicinal fungus that has various biological activities, including immunomodulatory and anti-tumor activities, the mechanisms of which are poorly understood. In the present study, we investigated the effects of mycelium extracts of P. linteus (MEPL) on the growth of human neuroblastoma SK-N-MC cells. Upon treatment with MEPL, a concentration-dependent inhibition of cell proliferation was observed and cells developed many of the hallmark features of apoptosis, including condensation of chromatin and an increase in the sub-G1 population. The anti-proliferative and apoptotic effects of MEPL were associated with a marked induction of the Bax and cyclin-dependent kinase inhibitor p21. Western blotting and in vitro caspase-3 activity assay demonstrated that the processing/activation of caspases accompanies the generation of MEPL-mediating apoptotic cell death. In addition, the proteolytic cleavage of specific target proteins such as poly(ADP-ribose) polymerase and β-catenin were observed. Taken together, the present results suggest that apoptotic signals evoked by MEPL in human neuroblastoma SK-N-MC cells may converge caspase-3 activation through an up-regulation of Bax rather than a down-regulation of Bcl-2.

Related Articles

Journal Cover

August 2004
Volume 14 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Choi YH, Huh MK, Ryu C, Choi BT and Jeong Y: Induction of apoptotic cell death by mycelium extracts of Phellinus linteus in human neuroblastoma cells. Int J Mol Med 14: 227-232, 2004
APA
Choi, Y.H., Huh, M.K., Ryu, C., Choi, B.T., & Jeong, Y. (2004). Induction of apoptotic cell death by mycelium extracts of Phellinus linteus in human neuroblastoma cells. International Journal of Molecular Medicine, 14, 227-232. https://doi.org/10.3892/ijmm.14.2.227
MLA
Choi, Y. H., Huh, M. K., Ryu, C., Choi, B. T., Jeong, Y."Induction of apoptotic cell death by mycelium extracts of Phellinus linteus in human neuroblastoma cells". International Journal of Molecular Medicine 14.2 (2004): 227-232.
Chicago
Choi, Y. H., Huh, M. K., Ryu, C., Choi, B. T., Jeong, Y."Induction of apoptotic cell death by mycelium extracts of Phellinus linteus in human neuroblastoma cells". International Journal of Molecular Medicine 14, no. 2 (2004): 227-232. https://doi.org/10.3892/ijmm.14.2.227