New experimental models for the in vitro reconstitution of human bladder mucosa

  • Authors:
    • Sergio De Angeli
    • Laura Del Pup
    • Andrea Fandella
    • Rosa Di Liddo
    • Giuseppe Anselmo
    • Carlo Macchi
    • Maria Teresa Conconi
    • Pier Paolo Parnigotto
    • Gastone G. Nussdorfer
  • View Affiliations

  • Published online on: September 1, 2004     https://doi.org/10.3892/ijmm.14.3.367
  • Pages: 367-372
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Abstract

This study describes two experimental models for the in vitro reconstitution of the human bladder mucosa (neo-bladder): human urothelial stabilized cell lines were cultured on three-dimensional matrices, collagen or platelet-fibrin gels, containing murine fibroblast 3T3-J2 cells. Low-density seeding (2x104 cells/ml) of both normal (TCA-48) and neoplastic cell lines (TCA-47) on collagen matrix gave rise to isolated papillar colonies, while high-density seeding (3.75x106 cells/ml) led to the formation of wide pluristratified epithelial sheets, resembling the normal transitional epithelium. In contrast, high-density seeding (5x105 cells/ml) on platelet-fibrin matrix did not allow the formation of epithelial sheets: only isolated voluminous colonies of normal TCA-48 cells, and sparse and small colonies of neoplastic TCA-47 could be observed. Growth assays and cytotoxicity reduction tests showed that the growth inhibitory effect of platelet-fibrin gel on urothelial cells was probably due to the aspecific activation of the complement contained in the plasmatic fraction, whose precipitation forms fibrin-glue. Collectively, these findings allow us to draw the following conclusions: i) neobladders obtained by culturing urothelial cells on collagen matrix reproduce normal bladder mucosa and could be utilized in pharmacological studies; and ii) platelet-fibrin gels, that specifically inhibit neoplastic urothelial cell growth, could be used as scaffolds in surgical bladder reconstitution.

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September 2004
Volume 14 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
De Angeli S, Del Pup L, Fandella A, Di Liddo R, Anselmo G, Macchi C, Conconi MT, Parnigotto PP and Nussdorfer GG: New experimental models for the in vitro reconstitution of human bladder mucosa. Int J Mol Med 14: 367-372, 2004
APA
De Angeli, S., Del Pup, L., Fandella, A., Di Liddo, R., Anselmo, G., Macchi, C. ... Nussdorfer, G.G. (2004). New experimental models for the in vitro reconstitution of human bladder mucosa. International Journal of Molecular Medicine, 14, 367-372. https://doi.org/10.3892/ijmm.14.3.367
MLA
De Angeli, S., Del Pup, L., Fandella, A., Di Liddo, R., Anselmo, G., Macchi, C., Conconi, M. T., Parnigotto, P. P., Nussdorfer, G. G."New experimental models for the in vitro reconstitution of human bladder mucosa". International Journal of Molecular Medicine 14.3 (2004): 367-372.
Chicago
De Angeli, S., Del Pup, L., Fandella, A., Di Liddo, R., Anselmo, G., Macchi, C., Conconi, M. T., Parnigotto, P. P., Nussdorfer, G. G."New experimental models for the in vitro reconstitution of human bladder mucosa". International Journal of Molecular Medicine 14, no. 3 (2004): 367-372. https://doi.org/10.3892/ijmm.14.3.367