Beacon[47-73] inhibits glucocorticoid secretion and growth of cultured rat and human adrenocortical cells

  • Authors:
    • Agnieszka Ziolkowska
    • Gianni Carraro
    • Piera Rebuffat
    • Raffaella Spinazzi
    • Gastone G. Nussdorfer
    • Marcin Rucinski
    • Ludwik K. Malendowicz
  • View Affiliations

  • Published online on: September 1, 2004     https://doi.org/10.3892/ijmm.14.3.457
  • Pages: 457-461
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Abstract

Evidence has been recently provided that beacon, an ubiquitin-like protein overexpressed in the hypothalamus of Israeli sand rat, is also expressed in several endocrine glands of the Wistar rat, including adrenal cortex. Moreover, it has been shown that the in vivo administration of beacon[47-73] (hereinafter, beacon) evokes within 60 min a marked decrease in the plasma concentrations of ACTH and corticosterone. Hence, we have investigated the effect of beacon (4x10−9 or 4x10−7 M) on the secretion and growth of cultured rat and human zona fasciculata/reticularis (ZF/R) cells. Reverse transcription-polymerase chain reaction detected beacon mRNA in all human adrenal cortexes examined. A 3-h exposure to beacon was ineffective, but prolonged (24 and 96 h) exposures significantly lowered basal corticosterone and cortisol secretion from cultured rat and human ZF/R cells, respectively. Moreover, beacon (4x10−7 M) counteracted the secretagogue action of 10−8 M ACTH on cultured cells. The 96-h exposure to beacon concentration-dependently decreased basal proliferation rate of cultured cells, without inducing significant changes in the number of apoptotic and necrotic cells. Beacon (4x10−7 M) significantly inhibited the proliferogenic effect of 10−8 M adrenomedullin. In light of the involvement of ubiquitin-like proteins in the control of cell cycle and protein sorting and degradation, the hypothesis is advanced that the inhibitory effect of beacon on the secretion and growth of cultured rat ZF/R cells may be connected to its stimulating effect on proteolysis of steroidogenic enzymes and proteins involved in cell replication.

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September 2004
Volume 14 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Ziolkowska A, Carraro G, Rebuffat P, Spinazzi R, Nussdorfer GG, Rucinski M and Malendowicz LK: Beacon[47-73] inhibits glucocorticoid secretion and growth of cultured rat and human adrenocortical cells. Int J Mol Med 14: 457-461, 2004
APA
Ziolkowska, A., Carraro, G., Rebuffat, P., Spinazzi, R., Nussdorfer, G.G., Rucinski, M., & Malendowicz, L.K. (2004). Beacon[47-73] inhibits glucocorticoid secretion and growth of cultured rat and human adrenocortical cells. International Journal of Molecular Medicine, 14, 457-461. https://doi.org/10.3892/ijmm.14.3.457
MLA
Ziolkowska, A., Carraro, G., Rebuffat, P., Spinazzi, R., Nussdorfer, G. G., Rucinski, M., Malendowicz, L. K."Beacon[47-73] inhibits glucocorticoid secretion and growth of cultured rat and human adrenocortical cells". International Journal of Molecular Medicine 14.3 (2004): 457-461.
Chicago
Ziolkowska, A., Carraro, G., Rebuffat, P., Spinazzi, R., Nussdorfer, G. G., Rucinski, M., Malendowicz, L. K."Beacon[47-73] inhibits glucocorticoid secretion and growth of cultured rat and human adrenocortical cells". International Journal of Molecular Medicine 14, no. 3 (2004): 457-461. https://doi.org/10.3892/ijmm.14.3.457