Effects of leptin and leptin fragments on corticosterone secretion and growth of cultured rat adrenocortical cells

  • Authors:
    • Ludwik K. Malendowicz
    • Raffaella Spinazzi
    • Cinzia Tortorella
    • Gastone G. Nussdorfer
    • Agnieszka Ziolkowska
    • Marcin Rucinski
  • View Affiliations

  • Published online on: November 1, 2004     https://doi.org/10.3892/ijmm.14.5.873
  • Pages: 873-877
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Abstract

Compelling evidence indicates that leptin, acting via specific receptors (Ob-Ra and Ob-Rb) modulates adrenocortical-cell secretion. However, the results are controversial, inasmuch as either secretagogue or antisecretagogue effects have been reported. Hence, we decided to study the effects of a 96-h incubation with leptin and leptin fragments 116-130, 150-167, 138-167, 93-105, 22-56 and 26-39 (10−8 and 10−6 M) on the secretion and growth of cultured rat adrenocortical cells. Reverse transcription-polymerase chain reaction showed that control cultures expressed both Ob-Ra and Ob-Rb isoforms. As expected, ACTH (10−8 M) raised corticosterone secretion and lowered proliferation rate of cultured cells. Native leptin elicited ACTH-like effects, while fragment 116-130 was ineffective. Leptin fragments 150-167 and 26-39 stimulated corticosterone production, and fragments 138-167 and 22-56 inhibited it. Fragment 93-105 exerted a dose-dependent biphasic effect on corticosterone secretion (i.e. stimulation and inhibition at the concentration of 10−8 and 10−6 M, respectively). Leptin fragment 26-39 enhanced proliferation of cultured cells, while fragments 138-167 and 22-56 were ineffective. Fragments 150-167 and 93-105 displayed proliferogenic and antiproliferogenic effects at the concentration of 10−8 and 10−6 M, respectively. Taken together, these findings allows us to conclude that native leptin and its fragments interact differently with Ob-Rs or interact with different Ob-R isoforms, thereby variously modulating secretion and growth of cultured rat adrenocortical cells.

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November 2004
Volume 14 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Malendowicz LK, Spinazzi R, Tortorella C, Nussdorfer GG, Ziolkowska A and Rucinski M: Effects of leptin and leptin fragments on corticosterone secretion and growth of cultured rat adrenocortical cells. Int J Mol Med 14: 873-877, 2004
APA
Malendowicz, L.K., Spinazzi, R., Tortorella, C., Nussdorfer, G.G., Ziolkowska, A., & Rucinski, M. (2004). Effects of leptin and leptin fragments on corticosterone secretion and growth of cultured rat adrenocortical cells. International Journal of Molecular Medicine, 14, 873-877. https://doi.org/10.3892/ijmm.14.5.873
MLA
Malendowicz, L. K., Spinazzi, R., Tortorella, C., Nussdorfer, G. G., Ziolkowska, A., Rucinski, M."Effects of leptin and leptin fragments on corticosterone secretion and growth of cultured rat adrenocortical cells". International Journal of Molecular Medicine 14.5 (2004): 873-877.
Chicago
Malendowicz, L. K., Spinazzi, R., Tortorella, C., Nussdorfer, G. G., Ziolkowska, A., Rucinski, M."Effects of leptin and leptin fragments on corticosterone secretion and growth of cultured rat adrenocortical cells". International Journal of Molecular Medicine 14, no. 5 (2004): 873-877. https://doi.org/10.3892/ijmm.14.5.873