Pathophysiological role of human β-defensins 2 in gastric mucosa

  • Authors:
    • Tadashi Ohara
    • Tetsuo Morishita
    • Hidekazu Suzuki
    • Tatsuhiro Masaoka
    • Hiroshi Nagata
    • Toshifumi Hibi
  • View Affiliations

  • Published online on: December 1, 2004     https://doi.org/10.3892/ijmm.14.6.1023
  • Pages: 1023-1027
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Abstract

Human β-defensins (HBDs) recognized in the stomach include HBD1, which is the constitutional human β-defensin (HBD), and HBD-2 and HBD-4, which are inducible HBDs. HBD-2 is an antimicrobial peptide that is involved in host defences against bacterial infections, such as Helicobacter pylori (H.pylori) in infection of the gastric mucosal epithelium. We examined the pathophysiological role of HBD-2, besides their roles as antimicrobial peptides. The materials used for the study consisted of gastric mucosal tissue specimens collected endoscopically from patients with conditions such as chronic gastritis associated with H. pylori infection, and gastric ulcers and gastritis due to non-steroidal anti-inflammatory drugs (NSAIDs) with or without H. pylori infection. We investigated the expression of HBD-2 and NF-κB by RT-PCR and immunoblotting, and the relation between the localization of HBD-2 and follicular dendritic cells (FDCs) by immunohistochemistry. Expression of HBD-2 was recognized in all the mucosal tissue specimens, irrespective of the presence or absence of H. pylori infection. All of the mucosal specimens expressing HBD-2 also revealed expression of NF-κB. In consecutive immunohisto-chemical staining, while expression of HBD-2 was observed in the gastric mucosal epithelium, FDCs were found to be localized in the lamina propria mucosae under the epithelial cell layer. These data suggested that in addition to being antimicrobial peptides, HBD-2 may also have a pathophysiological role as proinflammatory mediators, and that the HBD may act as proinflammatory mediators in concert with the dendritic cells (DC) by transmitting a signal from the mucosal surface to the lamina propria mucosae, which seems to be the original site of gastric mucosal damage.

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December 2004
Volume 14 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Ohara T, Morishita T, Suzuki H, Masaoka T, Nagata H and Hibi T: Pathophysiological role of human β-defensins 2 in gastric mucosa. Int J Mol Med 14: 1023-1027, 2004
APA
Ohara, T., Morishita, T., Suzuki, H., Masaoka, T., Nagata, H., & Hibi, T. (2004). Pathophysiological role of human β-defensins 2 in gastric mucosa. International Journal of Molecular Medicine, 14, 1023-1027. https://doi.org/10.3892/ijmm.14.6.1023
MLA
Ohara, T., Morishita, T., Suzuki, H., Masaoka, T., Nagata, H., Hibi, T."Pathophysiological role of human β-defensins 2 in gastric mucosa". International Journal of Molecular Medicine 14.6 (2004): 1023-1027.
Chicago
Ohara, T., Morishita, T., Suzuki, H., Masaoka, T., Nagata, H., Hibi, T."Pathophysiological role of human β-defensins 2 in gastric mucosa". International Journal of Molecular Medicine 14, no. 6 (2004): 1023-1027. https://doi.org/10.3892/ijmm.14.6.1023