2-O-methylisohemigossylic acid lactone, a sesquiterpene, isolated from roots of mokumen (Gossampinus malabarica) induces cell death and morphological change indicative of apoptotic chromatin condensation in human promyelotic leukemia HL-60 cells

  • Authors:
    • Hiroshige Hibasami
    • Kazumi Saitoh
    • Hirotaka Katsuzaki
    • Kunio Imai
    • Yue Aratanechemuge
    • Takashi Komiya
  • View Affiliations

  • Published online on: December 1, 2004     https://doi.org/10.3892/ijmm.14.6.1029
  • Pages: 1029-1033
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Abstract

2-O-methylisohemigossylic acid lactone, a sesquiterpene, was purified from roots of mokumen (Gossampinus malabarica) and identified by Mass, and 1H- and 13-NMR. This sesquiterpene displayed strong growth inhibitory effect against human promyelotic leukemia HL-60 cells. Apoptotic morphological change of the nucleus, including chromatin condensation was induced in the HL-60 cells treated with the sesquiterpene. The fragmentation of DNA by the sesquiterpene to oligonucleosomal-sized fragments, a characteristic of apoptosis, was observed to be dose- and time-dependent in the HL-60 cells. Inhibitors of caspases suppressed the DNA fragmentation induced by the sesquiterpene. These findings suggest that growth inhibition by the sesquiterpene of HL-60 cells results from the induction of apoptosis by the sesqui-terpene, and that caspase cascade is involved in the induction of apoptosis by the compound in the HL-60 cells.

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December 2004
Volume 14 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Hibasami H, Saitoh K, Katsuzaki H, Imai K, Aratanechemuge Y and Komiya T: 2-O-methylisohemigossylic acid lactone, a sesquiterpene, isolated from roots of mokumen (Gossampinus malabarica) induces cell death and morphological change indicative of apoptotic chromatin condensation in human promyelotic leukemia HL-60 cells. Int J Mol Med 14: 1029-1033, 2004
APA
Hibasami, H., Saitoh, K., Katsuzaki, H., Imai, K., Aratanechemuge, Y., & Komiya, T. (2004). 2-O-methylisohemigossylic acid lactone, a sesquiterpene, isolated from roots of mokumen (Gossampinus malabarica) induces cell death and morphological change indicative of apoptotic chromatin condensation in human promyelotic leukemia HL-60 cells. International Journal of Molecular Medicine, 14, 1029-1033. https://doi.org/10.3892/ijmm.14.6.1029
MLA
Hibasami, H., Saitoh, K., Katsuzaki, H., Imai, K., Aratanechemuge, Y., Komiya, T."2-O-methylisohemigossylic acid lactone, a sesquiterpene, isolated from roots of mokumen (Gossampinus malabarica) induces cell death and morphological change indicative of apoptotic chromatin condensation in human promyelotic leukemia HL-60 cells". International Journal of Molecular Medicine 14.6 (2004): 1029-1033.
Chicago
Hibasami, H., Saitoh, K., Katsuzaki, H., Imai, K., Aratanechemuge, Y., Komiya, T."2-O-methylisohemigossylic acid lactone, a sesquiterpene, isolated from roots of mokumen (Gossampinus malabarica) induces cell death and morphological change indicative of apoptotic chromatin condensation in human promyelotic leukemia HL-60 cells". International Journal of Molecular Medicine 14, no. 6 (2004): 1029-1033. https://doi.org/10.3892/ijmm.14.6.1029