Role of albumin in osteoblastic cells: Enhancement of cell proliferation and suppression of alkaline phosphatase activity

  • Authors:
    • Koichi Ishida
    • Masayoshi Yamaguchi
  • View Affiliations

  • Published online on: December 1, 2004     https://doi.org/10.3892/ijmm.14.6.1077
  • Pages: 1077-1081
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The role of albumin in osteoblastic cells was investigated. Osteoblastic MC3T3-E1 cells with subconfluent monolayers were cultured for 24 to 72 h in a medium without fetal bovine serum (FBS) in the presence or absence of albumin (0.25, 0.5, or 1.0 mg/ml of medium). The number of osteoblastic cells was significantly increased by culture for 24 to 72 h in the presence of albumin (1.0 mg/ml). The effect of albumin in increasing cell number was completely abolished in the presence of PD98049 (10−7 M), staurosporine (10−7 M), or dibucaine (10−7 M), which is an inhibitor of various protein kinases. Also, the stimulating effect of albumin on cell proliferation was completely prevented in the presence of cycloheximide (10−7 M), an inhibitor of protein synthesis, or 5,6-dichloro-1-β-D-ribofuranosylbenzimidazole (DRB), an inhibitor of transcriptional activity. DNA content was significantly increased in the cells cultured with albumin (1.0 mg/ml) addition for 24 to 72 h. Alkaline phosphatase activity in the cells, which participates in osteoblastic calcification, was significantly decreased by the culture with albumin (0.25, 0.5, or 1.0 mg/ml) for 24 to 72 h. The expression of insulin-like growth factor-I (IGF-I) and transforming growth factor-β1 mRNAs using transcription-polymerase chain reaction (RT-PCR) analysis in osteoblastic cells was not significantly altered by culture for 48 h with albumin (1.0 mg/ml). This study demonstrated that albumin had a role in the regulation of osteoblastic cell function.

Related Articles

Journal Cover

December 2004
Volume 14 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Ishida K and Ishida K: Role of albumin in osteoblastic cells: Enhancement of cell proliferation and suppression of alkaline phosphatase activity. Int J Mol Med 14: 1077-1081, 2004
APA
Ishida, K., & Ishida, K. (2004). Role of albumin in osteoblastic cells: Enhancement of cell proliferation and suppression of alkaline phosphatase activity. International Journal of Molecular Medicine, 14, 1077-1081. https://doi.org/10.3892/ijmm.14.6.1077
MLA
Ishida, K., Yamaguchi, M."Role of albumin in osteoblastic cells: Enhancement of cell proliferation and suppression of alkaline phosphatase activity". International Journal of Molecular Medicine 14.6 (2004): 1077-1081.
Chicago
Ishida, K., Yamaguchi, M."Role of albumin in osteoblastic cells: Enhancement of cell proliferation and suppression of alkaline phosphatase activity". International Journal of Molecular Medicine 14, no. 6 (2004): 1077-1081. https://doi.org/10.3892/ijmm.14.6.1077