Systematic search for natural antisense transcripts in eukaryotes (Review)

  • Authors:
    • Øystein Røsok
    • Mouldy Sioud
  • View Affiliations

  • Published online on: February 1, 2005     https://doi.org/10.3892/ijmm.15.2.197
  • Pages: 197-203
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Abstract

The availability of several complete genome sequences and large numbers of expressed sequences, has led to the development of bioinformatic strategies for large-scale predictions of natural antisense transcripts. In the past two years, this has given at least 1,600 human pairs and 2,500 murine pairs of natural antisense transcripts. However, due to limitations in bioinformatic search tools, experimental validation of the predicted antisense transcripts is crucial. This has been performed only for a small fraction of the large number of predicted natural antisense transcripts. Additionally, bioinformatic approaches will not allow systematic identification of natural antisense transcripts in specific cell or tissue types. More recently, an experimental approach for identification of sense-antisense transcript pairs has been developed. The principle of the strategy is based upon the selection of double-stranded cDNAs as a result of hybridization between first-strand cDNAs that should be generated in the presence of actinomycin D. This experimental strategy allows systematic analysis of sense and antisense partners in any cell or tissue type.

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February 2005
Volume 15 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Røsok Ø and Røsok Ø: Systematic search for natural antisense transcripts in eukaryotes (Review). Int J Mol Med 15: 197-203, 2005
APA
Røsok, Ø., & Røsok, Ø. (2005). Systematic search for natural antisense transcripts in eukaryotes (Review). International Journal of Molecular Medicine, 15, 197-203. https://doi.org/10.3892/ijmm.15.2.197
MLA
Røsok, Ø., Sioud, M."Systematic search for natural antisense transcripts in eukaryotes (Review)". International Journal of Molecular Medicine 15.2 (2005): 197-203.
Chicago
Røsok, Ø., Sioud, M."Systematic search for natural antisense transcripts in eukaryotes (Review)". International Journal of Molecular Medicine 15, no. 2 (2005): 197-203. https://doi.org/10.3892/ijmm.15.2.197