Prevention of tissue injury by ribbon antisense to TGF-β1 in the kidney

  • Authors:
    • Young-Kook Choi
    • Ik-Jae Moon
    • Hyun-Kyung Jung
    • Byung-Chul Jang
    • Seong-Il Seo
    • Jong-Gu Park
  • View Affiliations

  • Published online on: March 1, 2005     https://doi.org/10.3892/ijmm.15.3.391
  • Pages: 391-399
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Abstract

Transforming growth factor-beta1 (TGF-β1) is an important mediator of glomerulosclerosis and tubulointerstitial fibrosis in renal diseases. We designed ribbon-type antisense oligos of TGF-β1, TGF-β1 RiAS, and combined them with a short peptide of the nuclear localization signal to form a transfection complex of DNA/peptide/liposomes (DPL) for enhanced cellular uptake. When H4IIE cells were transfected with TGF-β1 RiAS, the level of TGF-β1 mRNA was reduced by >70%. We then examined the ratio of the kidney weight per body weight in rats. Whereas the weight ratio was 0.47% for the normal kidney, the ratio was 0.99% on day 5 after unilateral ureteric obstruction (UUO). The ratios were 0.95% with PBS injection, 1.07% with scrambled RiAS, and 0.68% with TGF-β1 RiAS. When examined for TGF-β1 expression in the tissue, the level of TGF-β1 mRNA was also significantly reduced following treatment with TGF-β1 RiAS. Further, physical changes such as diminished dilation, atrophy, as well as apoptosis caused by UUO were also found to be markedly reduced by TGF-β1 RiAS. The results show that ribbon antisense to TGF-β1 when combined with efficient uptake can effectively block TGF-β1 expression and preserve tissue integrity in kidneys with UUO.

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March 2005
Volume 15 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Choi Y, Moon I, Jung H, Jang B, Seo S and Park J: Prevention of tissue injury by ribbon antisense to TGF-β1 in the kidney. Int J Mol Med 15: 391-399, 2005
APA
Choi, Y., Moon, I., Jung, H., Jang, B., Seo, S., & Park, J. (2005). Prevention of tissue injury by ribbon antisense to TGF-β1 in the kidney. International Journal of Molecular Medicine, 15, 391-399. https://doi.org/10.3892/ijmm.15.3.391
MLA
Choi, Y., Moon, I., Jung, H., Jang, B., Seo, S., Park, J."Prevention of tissue injury by ribbon antisense to TGF-β1 in the kidney". International Journal of Molecular Medicine 15.3 (2005): 391-399.
Chicago
Choi, Y., Moon, I., Jung, H., Jang, B., Seo, S., Park, J."Prevention of tissue injury by ribbon antisense to TGF-β1 in the kidney". International Journal of Molecular Medicine 15, no. 3 (2005): 391-399. https://doi.org/10.3892/ijmm.15.3.391