Significance of increased proliferation of immature plasma cells in the appendix of patients with ulcerative colitis

  • Authors:
    • Tomohiro Kawachiya
    • Nobuhide Oshitani
    • Yoshio Jinno
    • Kenji Watanabe
    • Shiro Nakamura
    • Yasuhiro Fujiwara
    • Kazuhide Higuchi
    • Kiyoshi Maeda
    • Yukio Nishiguchi
    • Kosei Hirakawa
    • Takayuki Matsumoto
    • Tetsuo Arakawa
  • View Affiliations

  • Published online on: March 1, 2005     https://doi.org/10.3892/ijmm.15.3.417
  • Pages: 417-423
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The etiology of ulcerative colitis (UC) is not known. Recent studies support a primary role of the appendix in the pathogenesis of UC, however phenotypical studies of proliferating cells in the appendix have not been reported. We report phenotypical studies of lymphocytes and of proliferating subpopulations in the appendix of patients with inflammatory bowel disease and of controls. Surgical samples of the appendix were obtained from 5 patients with colon cancer, 5 with acute appendicitis, 12 with UC and 7 with Crohn's disease (CD). Frozen sections were cut from fixed samples, and immunostained with lymphocyte markers and anti-Ki-67 antibodies. The number of Ki-67+ proliferating cells, CD19, and CD138 cells was significantly higher in the appendix of patients with UC than in controls, patients with acute appendicitis, and patients with CD. Immunohistological double staining revealed significant proliferation of CD3, CD19, and CD138 cells in the appendix of patients with UC. The proportions of Ki-67+ cells in CD3, CD19, and CD138 cells were significantly higher in both total UC patients and patients in remission-stage UC, than in controls, patients with acute appendicitis, and patients with CD. Lamina propria cells in the appendix of patients with UC showed augmented proliferation with increased numbers of CD19 and CD138 cells. The number of CD3 cells was not significantly increased, but the proportion of proliferating CD3 cells was increased. An increased proportion of Ki-67+ cells in CD19 and CD138 cells represents proliferation of immature plasma cells in the appendix of patients with UC, and proliferation of such immature plasma cells was seen in both active- and remission-stage UC. Proliferation of immature plasma cells in the appendix of patients with UC suggests a primary role of humoral immune responses in the pathogenesis of UC.

Related Articles

Journal Cover

March 2005
Volume 15 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Kawachiya T, Oshitani N, Jinno Y, Watanabe K, Nakamura S, Fujiwara Y, Higuchi K, Maeda K, Nishiguchi Y, Hirakawa K, Hirakawa K, et al: Significance of increased proliferation of immature plasma cells in the appendix of patients with ulcerative colitis. Int J Mol Med 15: 417-423, 2005
APA
Kawachiya, T., Oshitani, N., Jinno, Y., Watanabe, K., Nakamura, S., Fujiwara, Y. ... Arakawa, T. (2005). Significance of increased proliferation of immature plasma cells in the appendix of patients with ulcerative colitis. International Journal of Molecular Medicine, 15, 417-423. https://doi.org/10.3892/ijmm.15.3.417
MLA
Kawachiya, T., Oshitani, N., Jinno, Y., Watanabe, K., Nakamura, S., Fujiwara, Y., Higuchi, K., Maeda, K., Nishiguchi, Y., Hirakawa, K., Matsumoto, T., Arakawa, T."Significance of increased proliferation of immature plasma cells in the appendix of patients with ulcerative colitis". International Journal of Molecular Medicine 15.3 (2005): 417-423.
Chicago
Kawachiya, T., Oshitani, N., Jinno, Y., Watanabe, K., Nakamura, S., Fujiwara, Y., Higuchi, K., Maeda, K., Nishiguchi, Y., Hirakawa, K., Matsumoto, T., Arakawa, T."Significance of increased proliferation of immature plasma cells in the appendix of patients with ulcerative colitis". International Journal of Molecular Medicine 15, no. 3 (2005): 417-423. https://doi.org/10.3892/ijmm.15.3.417