Histologic and genetic analysis of the gallbladder in patients with occult pancreatobiliary reflux

  • Authors:
    • Takao Itoi
    • Akihiko Tsuchida
    • Fumihide Itokawa
    • Atsushi Sofuni
    • Toshio Kurihara
    • Takayoshi Tsuchiya
    • Fuminori Moriyasu
    • Kazuhiko Kasuya
    • Hiromi Serizawa
  • View Affiliations

  • Published online on: March 1, 2005     https://doi.org/10.3892/ijmm.15.3.425
  • Pages: 425-430
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Abstract

Recent studies have suggested that pancreatobiliary reflux occurs not only in patients with pancreaticobiliary maljunction (PBM), but also in patients without PBM and thereby possibly causes biliary tract disease. In this study, we examined prospectively histological findings and genetic analysis in the non-cancerous epithelium of the gallbladder in patients with high biliary amylase levels and a normal pancreaticobiliary junction. Ki-67 L.I of non-cancerous epithelium was 14.4 and 3.5% in the high biliary amylase levels (HBA) group and the low bliliary amylase levels (LBA) group, respectively (p<0.01). There was no case showing p53 overexpression regardless of amylase levels of bile. COX-2 expression was detected in the cytoplasm of non-cancerous epithelium in 9 cases in the HBA group and 5 cases in the LBA group. The positive rate of COX-2 overexpression was significantly higher in the HBA group than in the LBA group (p<0.05). COX-2 overexpression cases showed higher Ki-67 L.I than COX-2 non-overexpression cases (21.2 vs. 7.9%, p<0.05). Mutations of the K-ras gene were detected in non-cancerous gallbladder epithelium in 3 cases, only in the HBA group. Patterns of K-ras mutation at codon 12 were GAT in two cases and GTT in one case. Three cases showing COX-2 overexpression also showed K-ras mutation. These three cases showing K-ras mutation had comparatively high cellular proliferative activity (28, 26 and 14%). In conclusion, our data suggest that occult pancreaticobiliary reflux, especially with high biliary amylase levels, represents an important risk factor for the development of gallbladder carcinoma as well as PBM, and it may be possible to detect patients with such high biliary amylase levels by ERCP.

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March 2005
Volume 15 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Itoi T, Tsuchida A, Itokawa F, Sofuni A, Kurihara T, Tsuchiya T, Moriyasu F, Kasuya K and Serizawa H: Histologic and genetic analysis of the gallbladder in patients with occult pancreatobiliary reflux. Int J Mol Med 15: 425-430, 2005
APA
Itoi, T., Tsuchida, A., Itokawa, F., Sofuni, A., Kurihara, T., Tsuchiya, T. ... Serizawa, H. (2005). Histologic and genetic analysis of the gallbladder in patients with occult pancreatobiliary reflux. International Journal of Molecular Medicine, 15, 425-430. https://doi.org/10.3892/ijmm.15.3.425
MLA
Itoi, T., Tsuchida, A., Itokawa, F., Sofuni, A., Kurihara, T., Tsuchiya, T., Moriyasu, F., Kasuya, K., Serizawa, H."Histologic and genetic analysis of the gallbladder in patients with occult pancreatobiliary reflux". International Journal of Molecular Medicine 15.3 (2005): 425-430.
Chicago
Itoi, T., Tsuchida, A., Itokawa, F., Sofuni, A., Kurihara, T., Tsuchiya, T., Moriyasu, F., Kasuya, K., Serizawa, H."Histologic and genetic analysis of the gallbladder in patients with occult pancreatobiliary reflux". International Journal of Molecular Medicine 15, no. 3 (2005): 425-430. https://doi.org/10.3892/ijmm.15.3.425