Heat shock protein 90 is required for increased DNA binding activity of activator protein-1, a heterodimer of Fos/JunD, in rheumatoid synovial cells under inflammatory stimuli

  • Authors:
    • Miki Murata
    • Yasushi Miura
    • Akira Hashiramoto
    • Hitomi Kitamura
    • Hiroki Kawasaki
    • Kazuko Shiozawa
    • Shinichi Yoshiya
    • Hisamitsu Baba
    • Kazuo Chihara
    • Shunichi Shiozawa
  • View Affiliations

  • Published online on: April 1, 2005     https://doi.org/10.3892/ijmm.15.4.649
  • Pages: 649-653
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Abstract

We have studied the DNA binding profiles of activator protein-1 (AP-1) involved in synovial overgrowth and osteoporosis in rheumatoid arthritis (RA) in relation to the molecular chaperon heat shock protein 90 (HSP90). The AP-1 binding activity of the nuclear extracts of rheumatoid synovial cells was basically increased as compared with osteoarthritic synovial cells. Upon stimulation with inflammatory cytokines IL-1β or TNFα, the AP-1 binding activity was further increased in rheumatoid synovial cells, and increased AP-1 protein was composed as heterodimers of Fos and JunD which was not known before as a major component of AP-1 in rheumatoid synovial cells. The increase of AP-1 binding activity as induced by inflammatory cytokines was specifically inhibited by geldanamycin, radicicol or herbimycin A, specific inhibitors of HSP90, while AP-1 protein was not decreased by geldanamycin. Further, HSP90 protein was not decreased by the inhibitors. The findings indicate that HSP90 is required for increased AP-1 binding activity of rheumatoid synovial cells under inflammatory stimuli and that AP-1 binding activity is inhibited by functionally inactivating HSP90 with the inhibitors.

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April 2005
Volume 15 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Murata M, Miura Y, Hashiramoto A, Kitamura H, Kawasaki H, Shiozawa K, Yoshiya S, Baba H, Chihara K, Shiozawa S, Shiozawa S, et al: Heat shock protein 90 is required for increased DNA binding activity of activator protein-1, a heterodimer of Fos/JunD, in rheumatoid synovial cells under inflammatory stimuli. Int J Mol Med 15: 649-653, 2005
APA
Murata, M., Miura, Y., Hashiramoto, A., Kitamura, H., Kawasaki, H., Shiozawa, K. ... Shiozawa, S. (2005). Heat shock protein 90 is required for increased DNA binding activity of activator protein-1, a heterodimer of Fos/JunD, in rheumatoid synovial cells under inflammatory stimuli. International Journal of Molecular Medicine, 15, 649-653. https://doi.org/10.3892/ijmm.15.4.649
MLA
Murata, M., Miura, Y., Hashiramoto, A., Kitamura, H., Kawasaki, H., Shiozawa, K., Yoshiya, S., Baba, H., Chihara, K., Shiozawa, S."Heat shock protein 90 is required for increased DNA binding activity of activator protein-1, a heterodimer of Fos/JunD, in rheumatoid synovial cells under inflammatory stimuli". International Journal of Molecular Medicine 15.4 (2005): 649-653.
Chicago
Murata, M., Miura, Y., Hashiramoto, A., Kitamura, H., Kawasaki, H., Shiozawa, K., Yoshiya, S., Baba, H., Chihara, K., Shiozawa, S."Heat shock protein 90 is required for increased DNA binding activity of activator protein-1, a heterodimer of Fos/JunD, in rheumatoid synovial cells under inflammatory stimuli". International Journal of Molecular Medicine 15, no. 4 (2005): 649-653. https://doi.org/10.3892/ijmm.15.4.649