Roles of His9 (P2 subsite) and His13 (P3' subsite) in angiotensinogen for catalytic reaction of renin

  • Authors:
    • A.H.M. Nurun Nabi
    • Mohammad Nasir Uddin
    • Tsutomu Nakagawa
    • Takenori Orihashi
    • Akio Ebihara
    • Atsushi Iwasawa
    • Yukio Nakamura
    • Fumiaki Suzuki
  • View Affiliations

  • Published online on: July 1, 2005     https://doi.org/10.3892/ijmm.16.1.103
  • Pages: 103-107
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Abstract

Roles of His9 (P2) and His13 (P3') of angiotensinogen for the catalytic reaction of renin were investigated using purified recombinant ovine angiotensinogen and its mutants H9Q and H13Q. The pH depended reaction of human renin with angiotensinogens of wild-type, H9Q and H13Q showed peaks at pH 6.5 and 8.5, but the altitude of each peak was different. The Vmax of the reaction between H9Q and H13Q with human renin was decreased by about 50 and 70%, respectively, in comparison to wild-type angiotensinogen, at pH 6.5. At pH 8.5, the Vmax of H9Q and H13Q was 50 and 100% of that of wild-type, respectively. At pH 6.5, it was revealed that the catalytic efficiency of renin (Vmax/Km) reduced to 20 and 60% after mutation of angiotensinogen at His9 and His13 with Gln, respectively. At pH 8.5, the catalytic efficiency decreased to 10 and 70% after these mutations, respectively. These findings, therefore, indicate that histidine residues at both P2 and P3' positions probably associate with the renin catalytic reaction for angiotensin I generation.

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July 2005
Volume 16 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Nabi AN, Uddin MN, Nakagawa T, Orihashi T, Ebihara A, Iwasawa A, Nakamura Y and Suzuki F: Roles of His9 (P2 subsite) and His13 (P3' subsite) in angiotensinogen for catalytic reaction of renin. Int J Mol Med 16: 103-107, 2005
APA
Nabi, A.N., Uddin, M.N., Nakagawa, T., Orihashi, T., Ebihara, A., Iwasawa, A. ... Suzuki, F. (2005). Roles of His9 (P2 subsite) and His13 (P3' subsite) in angiotensinogen for catalytic reaction of renin. International Journal of Molecular Medicine, 16, 103-107. https://doi.org/10.3892/ijmm.16.1.103
MLA
Nabi, A. N., Uddin, M. N., Nakagawa, T., Orihashi, T., Ebihara, A., Iwasawa, A., Nakamura, Y., Suzuki, F."Roles of His9 (P2 subsite) and His13 (P3' subsite) in angiotensinogen for catalytic reaction of renin". International Journal of Molecular Medicine 16.1 (2005): 103-107.
Chicago
Nabi, A. N., Uddin, M. N., Nakagawa, T., Orihashi, T., Ebihara, A., Iwasawa, A., Nakamura, Y., Suzuki, F."Roles of His9 (P2 subsite) and His13 (P3' subsite) in angiotensinogen for catalytic reaction of renin". International Journal of Molecular Medicine 16, no. 1 (2005): 103-107. https://doi.org/10.3892/ijmm.16.1.103