Generation and characterization of iBREC: Novel hTERT-immortalized bovine retinal endothelial cells

  • Authors:
    • Heidrun Deissler
    • Helmut Deissler
    • Gerhard K. Lang
    • Gabriele E. Lang
  • View Affiliations

  • Published online on: July 1, 2005     https://doi.org/10.3892/ijmm.16.1.65
  • Pages: 65-70
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Abstract

Primary retina-derived endothelial cells isolated from bovine and other animal tissues are extremely useful for studying the molecular mechanisms involved in the pathogenesis of diabetic retinopathy. Preparations of primary bovine retinal endothelial cells (BREC), however, usually contain a number of contaminating non-endothelial cells, e.g. pericytes which have been shown to inhibit proliferation of endothelial cells. To overcome this major drawback, we immortalized BREC by reconstitution of telomerase activity as a consequence of CMV promoter-driven expression of the catalytic domain of human telomerase. Immortal BREC (iBREC) have the capacity to proliferate for >90 passages over 10 months and constantly express endothelial marker proteins. In addition, iBREC behave like BREC with respect to stimulation of proliferation with VEGF, IGF-1 and bFGF, indicating that we have generated a stable endothelial cell line closely related to the corresponding primary cells.

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July 2005
Volume 16 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Deissler H, Deissler H, Lang GK and Lang GE: Generation and characterization of iBREC: Novel hTERT-immortalized bovine retinal endothelial cells. Int J Mol Med 16: 65-70, 2005
APA
Deissler, H., Deissler, H., Lang, G.K., & Lang, G.E. (2005). Generation and characterization of iBREC: Novel hTERT-immortalized bovine retinal endothelial cells. International Journal of Molecular Medicine, 16, 65-70. https://doi.org/10.3892/ijmm.16.1.65
MLA
Deissler, H., Deissler, H., Lang, G. K., Lang, G. E."Generation and characterization of iBREC: Novel hTERT-immortalized bovine retinal endothelial cells". International Journal of Molecular Medicine 16.1 (2005): 65-70.
Chicago
Deissler, H., Deissler, H., Lang, G. K., Lang, G. E."Generation and characterization of iBREC: Novel hTERT-immortalized bovine retinal endothelial cells". International Journal of Molecular Medicine 16, no. 1 (2005): 65-70. https://doi.org/10.3892/ijmm.16.1.65