Nuclear localization of a novel protein, RGPR-p117, in cloned normal rat kidney proximal tubular epithelial cells

  • Authors:
    • Natsumi Sawada
    • Taeko Nakagawa
    • Tomiyasu Murata
    • Masayoshi Yamaguchi
  • View Affiliations

  • Published online on: November 1, 2005     https://doi.org/10.3892/ijmm.16.5.809
  • Pages: 809-814
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

A novel protein RGPR-p117 was discovered as a regucalcin gene promoter region-related protein that binds to the TTGGC motif. The role of RGPR-p117 in cell function is unknown. The nuclear localization of RGPR-p117 was investigated using cloned normal rat kidney proximal tubular epithelial NRK52E cells in vitro. RGPR-p117 mRNA was expressed in NRK52E cells, and its expression was stimulated by culture with parathyroid hormone (10−7 M) or phorbol 12-myristate (10−6 M). RGPR-p117 was found to localize in the cytoplasm and nucleus with immunocytochemical and Western blot analysis using HA-RGPR-p117/phCMV2-transfected NRK52E cells. Overexpression of HA-RGPR-p117 was found to have a significant stimulatory effect on regucalcin mRNA expression in NRK52E cells. This study demonstrates that RGPR-p117 is localized in the nucleus of kidney cells, and may be involved in gene expression.

Related Articles

Journal Cover

November 2005
Volume 16 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Sawada N, Nakagawa T, Murata T and Yamaguchi M: Nuclear localization of a novel protein, RGPR-p117, in cloned normal rat kidney proximal tubular epithelial cells. Int J Mol Med 16: 809-814, 2005
APA
Sawada, N., Nakagawa, T., Murata, T., & Yamaguchi, M. (2005). Nuclear localization of a novel protein, RGPR-p117, in cloned normal rat kidney proximal tubular epithelial cells. International Journal of Molecular Medicine, 16, 809-814. https://doi.org/10.3892/ijmm.16.5.809
MLA
Sawada, N., Nakagawa, T., Murata, T., Yamaguchi, M."Nuclear localization of a novel protein, RGPR-p117, in cloned normal rat kidney proximal tubular epithelial cells". International Journal of Molecular Medicine 16.5 (2005): 809-814.
Chicago
Sawada, N., Nakagawa, T., Murata, T., Yamaguchi, M."Nuclear localization of a novel protein, RGPR-p117, in cloned normal rat kidney proximal tubular epithelial cells". International Journal of Molecular Medicine 16, no. 5 (2005): 809-814. https://doi.org/10.3892/ijmm.16.5.809