Role of protein kinase C in expression of granulocyte-colony stimulating factor and granulocyte macrophage-colony stimulating factor in lung cancer cells

  • Authors:
    • Yoshiki Uemura
    • Makoto Kobayashi
    • Hideshi Nakata
    • Tetsuya Kubota
    • Tsuyako Saito
    • Kentaro Bandobashi
    • Hirokuni Taguchi
  • View Affiliations

  • Published online on: November 1, 2005     https://doi.org/10.3892/ijmm.16.5.873
  • Pages: 873-881
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Abstract

We examine the role of protein kinase C (PKC) pathways in the constitutive expression of granulocyte-colony stimulating factor (G-CSF) and granulocyte macrophage-colony stimulating factor (GM-CSF) in lung cancer cells. Two cell lines, OKa-C-1 and MI-4, constitutively produce an abundant dose of G-CSF and GM-CSF. The PKC activator phorbol 12-myristate 13-acetate (PMA) stimulated the production of GM-CSF in a dose-dependent manner and reduced G-CSF in the cell lines. The PKC inhibitor staurosporine had effects opposite to those of PMA in the cell lines. Another PKC activator (4β-phorbol 12, 13-dibutyrate) and six specific PKC inhibitors (bisindolylmaleimide I, calphostin C, chelerythrine chloride, Gö 6976, PKC inhibitor 19-27, and Ro-32-0432) also worked as well as PMA and staurosporine, respectively. The induction of GM-CSF expression via PKC activation was mediated by the activation of nuclear factor-κB. The induction of G-CSF expression via PKC inhibition was mediated by p44/42 mitogen-activated protein kinase and c-Jun N-terminal kinase pathway signaling. GM-CSF may accelerate cell growth and inhibit cell death via PKC activation in the cell lines. G-CSF also seems to reverse growth suppression and cell death induced by PKC inhibition.

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November 2005
Volume 16 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Uemura Y, Kobayashi M, Nakata H, Kubota T, Saito T, Bandobashi K and Taguchi H: Role of protein kinase C in expression of granulocyte-colony stimulating factor and granulocyte macrophage-colony stimulating factor in lung cancer cells. Int J Mol Med 16: 873-881, 2005
APA
Uemura, Y., Kobayashi, M., Nakata, H., Kubota, T., Saito, T., Bandobashi, K., & Taguchi, H. (2005). Role of protein kinase C in expression of granulocyte-colony stimulating factor and granulocyte macrophage-colony stimulating factor in lung cancer cells. International Journal of Molecular Medicine, 16, 873-881. https://doi.org/10.3892/ijmm.16.5.873
MLA
Uemura, Y., Kobayashi, M., Nakata, H., Kubota, T., Saito, T., Bandobashi, K., Taguchi, H."Role of protein kinase C in expression of granulocyte-colony stimulating factor and granulocyte macrophage-colony stimulating factor in lung cancer cells". International Journal of Molecular Medicine 16.5 (2005): 873-881.
Chicago
Uemura, Y., Kobayashi, M., Nakata, H., Kubota, T., Saito, T., Bandobashi, K., Taguchi, H."Role of protein kinase C in expression of granulocyte-colony stimulating factor and granulocyte macrophage-colony stimulating factor in lung cancer cells". International Journal of Molecular Medicine 16, no. 5 (2005): 873-881. https://doi.org/10.3892/ijmm.16.5.873