Molecular characterization of the zinc finger transcription factor, Osterix

  • Authors:
    • Mitsutoki Hatta
    • Yoshitaka Yoshimura
    • Yoshiaki Deyama
    • Akiyoshi Fukamizu
    • Kuniaki Suzuki
  • View Affiliations

  • Published online on: March 1, 2006     https://doi.org/10.3892/ijmm.17.3.425
  • Pages: 425-430
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Abstract

Osterix was identified as a transcription factor expressing, in osteoblasts, required for bone formation. However, the molecular mechanisms of the gene regulation by Osterix remain elusive. In this study, we examined the transactivation property of Osterix by using the Gal4 fusion system reporter assay. We identified the transactivation domain of Osterix, which contains high proline and glycine residues and has an activation property in mammalian and yeast cells. The GST-pull down analysis revealed that the basal transcription factor, TF-IIB, but not TBP, binds to the transactivation domain. Furthermore, we found that Osterix interacts with chromatin remodeling factor, Brg-1, through its C-terminal zinc finger domain in vivo and in vitro. These findings suggest that Osterix possesses functional domains which associate with transcription mediated factors and functions as a transcriptional activator in the nucleus.

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March 2006
Volume 17 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Hatta M, Yoshimura Y, Deyama Y, Fukamizu A and Suzuki K: Molecular characterization of the zinc finger transcription factor, Osterix. Int J Mol Med 17: 425-430, 2006
APA
Hatta, M., Yoshimura, Y., Deyama, Y., Fukamizu, A., & Suzuki, K. (2006). Molecular characterization of the zinc finger transcription factor, Osterix. International Journal of Molecular Medicine, 17, 425-430. https://doi.org/10.3892/ijmm.17.3.425
MLA
Hatta, M., Yoshimura, Y., Deyama, Y., Fukamizu, A., Suzuki, K."Molecular characterization of the zinc finger transcription factor, Osterix". International Journal of Molecular Medicine 17.3 (2006): 425-430.
Chicago
Hatta, M., Yoshimura, Y., Deyama, Y., Fukamizu, A., Suzuki, K."Molecular characterization of the zinc finger transcription factor, Osterix". International Journal of Molecular Medicine 17, no. 3 (2006): 425-430. https://doi.org/10.3892/ijmm.17.3.425