RT-PCR and in situ hybridization analysis of apolipoprotein H expression in rat normal tissues

  • Authors:
    • Maria A. Ragusa
    • Salvatore Costa
    • Angelo B. Cefalù
    • Davide Noto
    • Francesca Fayer
    • Salvatore Travali
    • Maurizio R. Averna
    • Fabrizio Gianguzza
  • View Affiliations

  • Published online on: September 1, 2006     https://doi.org/10.3892/ijmm.18.3.449
  • Pages: 449-455
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Abstract

In this study, by using different techniques (i.e. Northern blot hybridization, RT-PCR and Southern blot hybridization) on various normal rat tissues, we were able to identify liver, kidney, heart, small intestine, brain, spleen, stomach and prostate as tissues in which the ApoH gene is transcribed. Moreover, for some of these tissues, by in situ hybridization, we found a specific localization of apoH transcripts. For instance epithelial cells of the bile ducts in liver and of the proximal tubules in kidney are the major sites of apoH synthesis. Our data suggest that some of the different physiological roles proposed for apoH could correlate with its direct expression, while others could correlate with its absorption from bloodstream or adjacent cells.

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September 2006
Volume 18 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Ragusa MA, Costa S, Cefalù AB, Noto D, Fayer F, Travali S, Averna MR and Gianguzza F: RT-PCR and in situ hybridization analysis of apolipoprotein H expression in rat normal tissues. Int J Mol Med 18: 449-455, 2006
APA
Ragusa, M.A., Costa, S., Cefalù, A.B., Noto, D., Fayer, F., Travali, S. ... Gianguzza, F. (2006). RT-PCR and in situ hybridization analysis of apolipoprotein H expression in rat normal tissues. International Journal of Molecular Medicine, 18, 449-455. https://doi.org/10.3892/ijmm.18.3.449
MLA
Ragusa, M. A., Costa, S., Cefalù, A. B., Noto, D., Fayer, F., Travali, S., Averna, M. R., Gianguzza, F."RT-PCR and in situ hybridization analysis of apolipoprotein H expression in rat normal tissues". International Journal of Molecular Medicine 18.3 (2006): 449-455.
Chicago
Ragusa, M. A., Costa, S., Cefalù, A. B., Noto, D., Fayer, F., Travali, S., Averna, M. R., Gianguzza, F."RT-PCR and in situ hybridization analysis of apolipoprotein H expression in rat normal tissues". International Journal of Molecular Medicine 18, no. 3 (2006): 449-455. https://doi.org/10.3892/ijmm.18.3.449