In utero transplantation of human hematopoietic stem/progenitor cells partially repairs injured liver in mice

  • Authors:
    • Hui Qian
    • Juan Wang
    • Shu Wang
    • Zhijuan Gong
    • Meijue Chen
    • Zhaorui Ren
    • Shuzhen Huang
  • View Affiliations

  • Published online on: October 1, 2006     https://doi.org/10.3892/ijmm.18.4.633
  • Pages: 633-642
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Abstract

The aim of this study is to establish a novel mouse model with high achievement and chimerism by in utero transplantation of human hematopoietic stem/progenitor cells and to explore the possibility that human adult hematopoietic stem/progenitor cells can differentiate into hepatocyte-like cells and partially repair the liver damage induced by carbon tetrachloride (CCl4). Mononuclear cells (MNCs) were isolated from fresh human umbilical cord blood (hUCB) and CD34+ cells were enriched from the MNCs by magnetic cell isolation. These cells were injected respectively into the fetal mice at 11-13 days of gestation. At one month after birth, the specific markers of human cells, human α-satellite sequence (h17α), CD14, CD34, CD45, and GPA were detected by PCR and FACS. At three and six months after birth, the established human-mouse chimeras were administered with CCl4 by intraperitoneal injection. The biochemical markers (ALT, AST, ALP, albumin) in serum were determined and human hepatocyte-specific proteins, such as human albumin, hepatocyte nuclear factor-4, hepatocyte-specific antigen, tryptophan 2,3-dioxygenase and α fetoprotein were analyzed by PCR, RT-PCR, real-time PCR and immunohistochemistry staining, respectively. More than 77% of recipients demonstrated human-mouse chimera. Significantly, hUCB hematopoietic stem/progenitor cells may differentiate into human hepatocyte-like cells with evidence of the expression of human hepatocyte-specific proteins as well as partially repair or protect liver damage induced by CCl4. The mouse model described in this article provides a useful tool for the studies of regeneration of human hepatocyte-like cells from adult hematopoietic stem/ progenitor cells as well as facilitates the therapeutic potential for liver diseases or damage by in utero transplantation.

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October 2006
Volume 18 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Qian H, Wang J, Wang S, Gong Z, Chen M, Ren Z and Huang S: In utero transplantation of human hematopoietic stem/progenitor cells partially repairs injured liver in mice. Int J Mol Med 18: 633-642, 2006
APA
Qian, H., Wang, J., Wang, S., Gong, Z., Chen, M., Ren, Z., & Huang, S. (2006). In utero transplantation of human hematopoietic stem/progenitor cells partially repairs injured liver in mice. International Journal of Molecular Medicine, 18, 633-642. https://doi.org/10.3892/ijmm.18.4.633
MLA
Qian, H., Wang, J., Wang, S., Gong, Z., Chen, M., Ren, Z., Huang, S."In utero transplantation of human hematopoietic stem/progenitor cells partially repairs injured liver in mice". International Journal of Molecular Medicine 18.4 (2006): 633-642.
Chicago
Qian, H., Wang, J., Wang, S., Gong, Z., Chen, M., Ren, Z., Huang, S."In utero transplantation of human hematopoietic stem/progenitor cells partially repairs injured liver in mice". International Journal of Molecular Medicine 18, no. 4 (2006): 633-642. https://doi.org/10.3892/ijmm.18.4.633