Protection of aortic endothelial function in both normal and diabetic rats by intravenous bolus injection of a medium-chain triglyceride: fish oil emulsion

  • Authors:
    • David Fontaine
    • Anne Otto
    • Laurence Portois
    • Jeanine Fontaine
    • Guy Berkenboom
    • Willy J. Malaisse
    • Yvon A. Carpentier
  • View Affiliations

  • Published online on: October 1, 2006     https://doi.org/10.3892/ijmm.18.4.697
  • Pages: 697-704
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Abstract

The bolus intravenous injection of a novel medium-chain triglyceride:fish oil emulsion (MCT:FO) was recently proposed as a tool to provoke a rapid and sustained increase of cell phospholipid content in long-chain polyunsaturated ω3 fatty acids, for instance in selected subjects prior to anesthesia and surgery. In this study, therefore, the possible protective effect of MCT:FO upon aortic endothelial function was investigated in both normal and diabetic rats. The animals were injected intravenously 20 h before sacrifice with 1.0 ml of either saline, MCT:FO or a control medium-chain triglyceride:long-chain triglyceride emulsion. The vasomotor response of isolated aortic rings was then explored by assessing the relaxation provoked by increasing concentrations of acetylcholine in rings contracted with phenylephrine. Such measurements were performed before and after exposure of the aortic rings to suitable concentrations of oxidized LDL. In both normal and diabetic rats, the prior injection of the MCT:FO emulsion protected the aortic rings against the deleterious effect of oxidized LDL. In the diabetic rats, a beneficial effect of the MCT:FO emulsion was even observed prior to exposure of the aortic rings to oxidized LDL. These findings support the view that this novel procedure is indeed appropriate to protect endothelial function against oxidative stress.

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October 2006
Volume 18 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Fontaine D, Otto A, Portois L, Fontaine J, Berkenboom G, Malaisse WJ and Carpentier YA: Protection of aortic endothelial function in both normal and diabetic rats by intravenous bolus injection of a medium-chain triglyceride: fish oil emulsion. Int J Mol Med 18: 697-704, 2006
APA
Fontaine, D., Otto, A., Portois, L., Fontaine, J., Berkenboom, G., Malaisse, W.J., & Carpentier, Y.A. (2006). Protection of aortic endothelial function in both normal and diabetic rats by intravenous bolus injection of a medium-chain triglyceride: fish oil emulsion. International Journal of Molecular Medicine, 18, 697-704. https://doi.org/10.3892/ijmm.18.4.697
MLA
Fontaine, D., Otto, A., Portois, L., Fontaine, J., Berkenboom, G., Malaisse, W. J., Carpentier, Y. A."Protection of aortic endothelial function in both normal and diabetic rats by intravenous bolus injection of a medium-chain triglyceride: fish oil emulsion". International Journal of Molecular Medicine 18.4 (2006): 697-704.
Chicago
Fontaine, D., Otto, A., Portois, L., Fontaine, J., Berkenboom, G., Malaisse, W. J., Carpentier, Y. A."Protection of aortic endothelial function in both normal and diabetic rats by intravenous bolus injection of a medium-chain triglyceride: fish oil emulsion". International Journal of Molecular Medicine 18, no. 4 (2006): 697-704. https://doi.org/10.3892/ijmm.18.4.697