Proteomic analysis of rat cerebral cortex, hippocampus and striatum after exposure to morphine

  • Authors:
    • Anna Bierczynska-Krzysik
    • Julius Paul Pradeep John
    • Jerzy Silberring
    • Jolanta Kotlinska
    • Tomasz Dylag
    • Maureen Cabatic
    • Gert Lubec
  • View Affiliations

  • Published online on: October 1, 2006     https://doi.org/10.3892/ijmm.18.4.775
  • Pages: 775-784
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Abstract

Although a series of proteins in the brain have been shown to be qualitatively or quantitatively dysregulated following morphine administration, a systematic proteomic study has not been carried out so far. We therefore aimed to show the effect of morphine on protein levels in the rat brain. For this purpose rats were given a morphine base in subcutaneously placed pellets and subsequently the cerebral cortex, hippocampus and striatum were taken for proteomic studies after three days. Extracted proteins were run on two-dimensional gel electrophoresis, scanned and quantified by specific software. Proteins with significantly different levels were analysed by mass spectrometry (MALDI-TOF-TOF). Twenty-six proteins were found to be differentially expressed and were unambiguously identified. Dysregulated proteins were from several protein pathways and cascades including signaling, metabolic, protein handling, antioxidant and miscellaneous classes. These findings represent an initial approach to the generation of a ‘morphinome’ and may form the basis for further protein chemical studies as a valuable analytical tool. Moreover, the study reveals morphine-regulated proteins in different brain areas and indicates the pathways involved following morphine administration in the rat, the main species for pharmacological studies in the field.

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October 2006
Volume 18 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Bierczynska-Krzysik A, Pradeep John JP, Silberring J, Kotlinska J, Dylag T, Cabatic M and Lubec G: Proteomic analysis of rat cerebral cortex, hippocampus and striatum after exposure to morphine. Int J Mol Med 18: 775-784, 2006
APA
Bierczynska-Krzysik, A., Pradeep John, J.P., Silberring, J., Kotlinska, J., Dylag, T., Cabatic, M., & Lubec, G. (2006). Proteomic analysis of rat cerebral cortex, hippocampus and striatum after exposure to morphine. International Journal of Molecular Medicine, 18, 775-784. https://doi.org/10.3892/ijmm.18.4.775
MLA
Bierczynska-Krzysik, A., Pradeep John, J. P., Silberring, J., Kotlinska, J., Dylag, T., Cabatic, M., Lubec, G."Proteomic analysis of rat cerebral cortex, hippocampus and striatum after exposure to morphine". International Journal of Molecular Medicine 18.4 (2006): 775-784.
Chicago
Bierczynska-Krzysik, A., Pradeep John, J. P., Silberring, J., Kotlinska, J., Dylag, T., Cabatic, M., Lubec, G."Proteomic analysis of rat cerebral cortex, hippocampus and striatum after exposure to morphine". International Journal of Molecular Medicine 18, no. 4 (2006): 775-784. https://doi.org/10.3892/ijmm.18.4.775