Interferon-ç induces cathepsin B expression in a human macrophage-like cell line by increasing both transcription and mRNA stability.

  • Authors:
    • Q Li
    • L Ding
    • CT Bever
  • View Affiliations

  • Published online on: August 1, 1998     https://doi.org/10.3892/ijmm.2.2.181
  • Pages: 181-367
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Abstract

We have demonstrated previously that treatment of the phorbol ester PMA-primed THP-1 human macrophage-like cells with interferon-ç (IFN-ç) in vitro induced time and dose-dependent increases in steady-state levels of cathepsin B (CB) mRNA. The present study was undertaken to investigate the mechanism of that increase. In vitro nuclear transcription (nuclear run-off) assays of CB gene expression were performed with purified nuclei from IFN-ç-treated or untreated THP-1 cells. These assays showed transcription to be increased approximately three-fold by IFN-ç in the PMA-primed THP-1 cells. Studies with á-amanitin indicated that the half-life of CB mRNA is prolonged after PMA and IFN-ç treatment, by more than 90%. Therefore, the elevated CB mRNA level results from a combination of IFN-ç-induced increase in the transcription rate of the CB gene and stabilization of the corresponding transcripts. The IFN-ç-mediated increase in CB gene transcription and steady-state mRNA level was blocked by á-amanitin or cycloheximide, suggesting the involvement of RNA polymerase II and the requirement of de novo protein synthesis.

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Aug 1998
Volume 2 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Li Q, Ding L and Bever C: Interferon-ç induces cathepsin B expression in a human macrophage-like cell line by increasing both transcription and mRNA stability.. Int J Mol Med 2: 181-367, 1998
APA
Li, Q., Ding, L., & Bever, C. (1998). Interferon-ç induces cathepsin B expression in a human macrophage-like cell line by increasing both transcription and mRNA stability.. International Journal of Molecular Medicine, 2, 181-367. https://doi.org/10.3892/ijmm.2.2.181
MLA
Li, Q., Ding, L., Bever, C."Interferon-ç induces cathepsin B expression in a human macrophage-like cell line by increasing both transcription and mRNA stability.". International Journal of Molecular Medicine 2.2 (1998): 181-367.
Chicago
Li, Q., Ding, L., Bever, C."Interferon-ç induces cathepsin B expression in a human macrophage-like cell line by increasing both transcription and mRNA stability.". International Journal of Molecular Medicine 2, no. 2 (1998): 181-367. https://doi.org/10.3892/ijmm.2.2.181