Telomere length, telomerase activity and telomerase RNA expression during mouse mammary tumor progression.

  • Authors:
    • Y Kiyozuka
    • A Asai
    • H Senzaki
    • Y Uemura
    • A Nakashima
    • J Morimoto
    • A Matsuzawa
    • A Tsubura
  • View Affiliations

  • Published online on: October 1, 1998     https://doi.org/10.3892/ijmm.2.4.437
  • Pages: 437-481
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Abstract

To investigate the roles of telomere length (mean length of the terminal restriction fragments; TRFs), telomerase activity (TA) and telomerase RNA (mTR) expression in relation to mouse mammary tumor progression, we examined a pregnancy-dependent mouse mammary tumor line (TPDMT-4) and its four autonomous sublines (T4-OI320: non-metastatic; and T4-OI165, -OI96, and -OI145: artificial metastatic) of DDD/1 mouse origin, and an autonomous growing mammary tumor (JYG-MC) showing spontaneous lung metastasis developed in BALB/c mice infected with a Chinese feral mice (Sub-Jyg)-derived mouse mammary tumor virus (JYG-MTV). Compared with normal (pregnant) mammary tissue, the TA was elevated in the TPDMT-4 tumor and in the non-metastatic subline tumor (T4-OI320) (x10 fold, respectively), and was further increased (x13-15 fold) in parallel with the acquisition of metastatic potential (T4-OI165, -OI96, and -OI145). The mTR level was upregulated (x2.7-2.8 fold) in all autonomous growing tumors compared to the normal counter-part, but not in TPDMT-4. The TRF was shorter in accord with tumor progression (normal mammary tissue, 48 kb; TPDMT-4, 45 kb; T4-OI320, 37 kb; T4-OI165, -OI96 and -OI145, mean 37.7 kb; and JYG-MC, 21 kb). These results suggest that the activation of TA occurs as an early event at the stage of hormone-dependent tumorigenesis, followed by the up-regulation of mTR expression in accordance with the acquisition of autonomous growth, and then further activation of TA occurs when the tumor acquires metastatic potential. The TRF shortening was in parallel with the tumor progression.

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Oct 1998
Volume 2 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Kiyozuka Y, Asai A, Senzaki H, Uemura Y, Nakashima A, Morimoto J, Matsuzawa A and Tsubura A: Telomere length, telomerase activity and telomerase RNA expression during mouse mammary tumor progression.. Int J Mol Med 2: 437-481, 1998
APA
Kiyozuka, Y., Asai, A., Senzaki, H., Uemura, Y., Nakashima, A., Morimoto, J. ... Tsubura, A. (1998). Telomere length, telomerase activity and telomerase RNA expression during mouse mammary tumor progression.. International Journal of Molecular Medicine, 2, 437-481. https://doi.org/10.3892/ijmm.2.4.437
MLA
Kiyozuka, Y., Asai, A., Senzaki, H., Uemura, Y., Nakashima, A., Morimoto, J., Matsuzawa, A., Tsubura, A."Telomere length, telomerase activity and telomerase RNA expression during mouse mammary tumor progression.". International Journal of Molecular Medicine 2.4 (1998): 437-481.
Chicago
Kiyozuka, Y., Asai, A., Senzaki, H., Uemura, Y., Nakashima, A., Morimoto, J., Matsuzawa, A., Tsubura, A."Telomere length, telomerase activity and telomerase RNA expression during mouse mammary tumor progression.". International Journal of Molecular Medicine 2, no. 4 (1998): 437-481. https://doi.org/10.3892/ijmm.2.4.437