Measurement of circulating immunoreactive alpha-atrial natriuretic peptide in human plasma: validation and clinical application.

  • Authors:
    • A Ationu
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  • Published online on: October 1, 1998     https://doi.org/10.3892/ijmm.2.4.497
  • Pages: 497-997
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Abstract

Recent evidence suggests that plasma alpha-atrial natriuretic peptide (alpha-ANP) may serve as a useful biochemical marker of severe heart failure, as circulating levels become ighly elevated. In the present study, a specific radioimmunoassay (RIA) protocol was adapted and optimised for rapid quantitative measurement of circulating alpha-ANP levels in plasma taken from clinically normal subjects and heart transplant recipients with no evidence of heart failure or heart transplant rejection. The plasma concentration of immunoreactive alpha-ANP in heart transplant recipients (115 +/- 10 pg/ml, n = 14) was significantly higher (p < 0.01) than in normal subjects (14 +/- 5 pg/ml, n = 20). Comparison of circulating plasma immunoreactive alpha-ANP levels obtained by the adapted assay to levels obtained by a standard RIA protocol revealed a significant correlation (r = 0.998, y = 1.004 x -2.94, n = 40). The adapted assay is simple, precise, and with a shorter incubation time that would enable results to be available within one working day. These results show that circulating biological active a-ANP levels are elevated in healthy heart transplant recipients with no evidence of heart failure; thus extending previous reports concerning the elevation in circulating alpha-ANP levels from patients with a wide range of heart disorders. The adapted RIA protocol would facilitate rapid detection and quantification of alpha-ANP in experimental research and routine clinical investigations.

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Oct 1998
Volume 2 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Ationu A: Measurement of circulating immunoreactive alpha-atrial natriuretic peptide in human plasma: validation and clinical application.. Int J Mol Med 2: 497-997, 1998
APA
Ationu, A. (1998). Measurement of circulating immunoreactive alpha-atrial natriuretic peptide in human plasma: validation and clinical application.. International Journal of Molecular Medicine, 2, 497-997. https://doi.org/10.3892/ijmm.2.4.497
MLA
Ationu, A."Measurement of circulating immunoreactive alpha-atrial natriuretic peptide in human plasma: validation and clinical application.". International Journal of Molecular Medicine 2.4 (1998): 497-997.
Chicago
Ationu, A."Measurement of circulating immunoreactive alpha-atrial natriuretic peptide in human plasma: validation and clinical application.". International Journal of Molecular Medicine 2, no. 4 (1998): 497-997. https://doi.org/10.3892/ijmm.2.4.497