Microarray analysis of insulin-regulated gene expression in the liver: The use of transgenic mice co-expressing insulin-siRNA and human IDE as an animal model

  • Authors:
    • Seungwan Jee
    • Daeyoun Hwang
    • Sujin Seo
    • Yongkyu Kim
    • Chuelkyu Kim
    • Byungguk Kim
    • Sunbo Shim
    • Suhae Lee
    • Jisoon Sin
    • Changjun Bae
    • Byoungchun Lee
    • Meekyung Jang
    • Minsun Kim
    • Suyoun Yim
    • Insurk Jang
    • Jungsik Cho
    • Kabryong Chae
  • View Affiliations

  • Published online on: December 1, 2007     https://doi.org/10.3892/ijmm.20.6.829
  • Pages: 829-835
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

To characterize the changes in global gene expression in the livers of H1/siRNAinsulin-CMV/hIDE transgenic (Tg) mice in response to the reduced bioavailability of insulin, total RNA extracted from the livers of 20-week-old Tg and non-Tg mice was converted to cDNA, labeled with biotin and hybridized to oligonucleotide microarrays. The microarray results were confirmed by a real-time reverse transcription-polymerase chain reaction. Two hundred and fifty-one and 73 genes were up- and down-regulated, respectively by insulin in H1/siRNAinsulin-CMV/hIDE Tg mice compared to the controls. Genes encoding for physiological processes, extracellular defense response and response to biotic stimuli were significantly over-represented in the up-regulated group. Among the down-regulated transcripts, those encoding for extracellular matrix proteins were dramatically over-represented, followed by those related to monooxygenase and oxidoreductase activities. The major genes in the up-regulated categories included Egr1, Saa2, Atf3, DNAJB1 and cCL2, whereas those in the down-regulated categories were Cyp17a1, Adn, Gadd45g, Eno3 and Moxd1. These results indicate that the microarray analysis identifies several gene functional groups and individual genes that respond to a sustained reduction in the insulin levels in the livers of Tg mice. These results also suggest that microarray testing is a useful tool for the better understanding of insulin-regulated diabetes-related diseases.

Related Articles

Journal Cover

December 2007
Volume 20 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Jee S, Hwang D, Seo S, Kim Y, Kim C, Kim B, Shim S, Lee S, Sin J, Bae C, Bae C, et al: Microarray analysis of insulin-regulated gene expression in the liver: The use of transgenic mice co-expressing insulin-siRNA and human IDE as an animal model. Int J Mol Med 20: 829-835, 2007
APA
Jee, S., Hwang, D., Seo, S., Kim, Y., Kim, C., Kim, B. ... Chae, K. (2007). Microarray analysis of insulin-regulated gene expression in the liver: The use of transgenic mice co-expressing insulin-siRNA and human IDE as an animal model. International Journal of Molecular Medicine, 20, 829-835. https://doi.org/10.3892/ijmm.20.6.829
MLA
Jee, S., Hwang, D., Seo, S., Kim, Y., Kim, C., Kim, B., Shim, S., Lee, S., Sin, J., Bae, C., Lee, B., Jang, M., Kim, M., Yim, S., Jang, I., Cho, J., Chae, K."Microarray analysis of insulin-regulated gene expression in the liver: The use of transgenic mice co-expressing insulin-siRNA and human IDE as an animal model". International Journal of Molecular Medicine 20.6 (2007): 829-835.
Chicago
Jee, S., Hwang, D., Seo, S., Kim, Y., Kim, C., Kim, B., Shim, S., Lee, S., Sin, J., Bae, C., Lee, B., Jang, M., Kim, M., Yim, S., Jang, I., Cho, J., Chae, K."Microarray analysis of insulin-regulated gene expression in the liver: The use of transgenic mice co-expressing insulin-siRNA and human IDE as an animal model". International Journal of Molecular Medicine 20, no. 6 (2007): 829-835. https://doi.org/10.3892/ijmm.20.6.829