Pseudomonas fluorescens proliferates in a mouse organ homogenate at low temperature

  • Authors:
    • Yota Tatara
    • Takahiro Terakawa
    • Youhei Yamagata
    • Takafumi Uchida
  • View Affiliations

  • Published online on: May 1, 2008     https://doi.org/10.3892/ijmm.21.5.621
  • Pages: 621-626
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

In this study we observed the proliferation of Pseudomonas fluorescens (P. fluorescens) in mouse organ homogenates at 4°C. P. fluorescens secreted a protease possessing properties different from those of the mammalian tissue proteases. The specificity of this protease required a basic amino acid residue at the P1 position at a pH optimum of 6.0. The specificity of the protease was similar to that of trypsin, but the pH optimum was different. The protease mildly degraded elastin-Congo red; this suggests that the protease serves as an alternative for elastase in the case of P. fluorescens strains that lack virulent elastase. The protease was identified as an alkaline protease of P. fluorescens by liquid chromatography-tandem mass spectrometry analysis. Our results show that proteome analysis of the soluble proteins is useful in identifying bacterial species, particularly the bacterial contaminants in samples containing antibiotics.

Related Articles

Journal Cover

May 2008
Volume 21 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Tatara Y, Terakawa T, Yamagata Y and Uchida T: Pseudomonas fluorescens proliferates in a mouse organ homogenate at low temperature. Int J Mol Med 21: 621-626, 2008
APA
Tatara, Y., Terakawa, T., Yamagata, Y., & Uchida, T. (2008). Pseudomonas fluorescens proliferates in a mouse organ homogenate at low temperature. International Journal of Molecular Medicine, 21, 621-626. https://doi.org/10.3892/ijmm.21.5.621
MLA
Tatara, Y., Terakawa, T., Yamagata, Y., Uchida, T."Pseudomonas fluorescens proliferates in a mouse organ homogenate at low temperature". International Journal of Molecular Medicine 21.5 (2008): 621-626.
Chicago
Tatara, Y., Terakawa, T., Yamagata, Y., Uchida, T."Pseudomonas fluorescens proliferates in a mouse organ homogenate at low temperature". International Journal of Molecular Medicine 21, no. 5 (2008): 621-626. https://doi.org/10.3892/ijmm.21.5.621