Phosphoproteomic analysis of aged skeletal muscle

  • Authors:
    • Joan Gannon
    • Lisa Staunton
    • Kathleen O'Connell
    • Philip Doran
    • Kay Ohlendieck
  • View Affiliations

  • Published online on: July 1, 2008     https://doi.org/10.3892/ijmm.22.1.33
  • Pages: 33-42
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Abstract

One of the most important post-translational modifications is represented by phosphorylation on tyrosine, threonine and serine residues. Since abnormal phosphorylation is associated with various pathologies, it was of interest to perform a phosphoproteomic profiling of age-related skeletal muscle degeneration. We used the fluorescent phospho-specific Pro-Q Diamond dye to determine whether changes in the overall phosphorylation of the soluble skeletal muscle proteome differs significantly between young adult and senescent fibres. As an established model system of sarcopenia, we employed 30-month-old rat gastrocnemius fibres. Following the mass spectrometric identification of 59 major 2-D phosphoprotein landmark spots, the fluorescent dye staining survey revealed that 22 muscle proteins showed a differential expression pattern between 3-month- and 30-month-old muscle. Increased phosphorylation levels were shown for myosin light chain 2, tropomyosin α, lactate dehydrogenase, desmin, actin, albumin and aconitase. In contrast, decreased phospho-specific dye binding was observed for cytochrome c oxidase, creatine kinase and enolase. Thus, aging-induced alterations in phosphoproteins appear to involve the contractile machinery and the cytoskeleton, as well as the cytosolic and mitochondrial metabolism. This confirms that sarcopenia of old age is a complex neuromuscular pathology that is associated with drastic changes in the abundance and structure of key muscle proteins.

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July 2008
Volume 22 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Gannon J, Staunton L, O'Connell K, Doran P and Ohlendieck K: Phosphoproteomic analysis of aged skeletal muscle. Int J Mol Med 22: 33-42, 2008
APA
Gannon, J., Staunton, L., O'Connell, K., Doran, P., & Ohlendieck, K. (2008). Phosphoproteomic analysis of aged skeletal muscle. International Journal of Molecular Medicine, 22, 33-42. https://doi.org/10.3892/ijmm.22.1.33
MLA
Gannon, J., Staunton, L., O'Connell, K., Doran, P., Ohlendieck, K."Phosphoproteomic analysis of aged skeletal muscle". International Journal of Molecular Medicine 22.1 (2008): 33-42.
Chicago
Gannon, J., Staunton, L., O'Connell, K., Doran, P., Ohlendieck, K."Phosphoproteomic analysis of aged skeletal muscle". International Journal of Molecular Medicine 22, no. 1 (2008): 33-42. https://doi.org/10.3892/ijmm.22.1.33