Expression of PPARγ is reduced by medium supplementation with L-glutamine in human colorectal Caco-2 cells

  • Authors:
    • Cathy Fiatte
    • Cécile Huin
    • Philippe Collet
    • François Plénat
    • Michel Dauça
    • Hervé Schohn
  • View Affiliations

  • Published online on: December 1, 2008     https://doi.org/10.3892/ijmm_00000091
  • Pages: 825-832
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Abstract

Peroxisome proliferator-activated receptor-γ (PPARγ) belongs to the nuclear hormone receptor family. This receptor is implicated in colon cell differentiation and in colon cancer. Receptor activation by specific agonists has been shown to protect against colon cancer progression. PPARγ protein content within cells is modulated by several mechanisms, including proteasome degradation, activation of Wnt signalling pathways and presence of fermentation products such as butyrate. Herein, we investigated the impact of L-glutamine on PPARγ expression during the differentiation of Caco-2 cells grown in medium containing dialyzed fetal calf serum supplemented or not with L-glutamine. Using RT-PCR and Western blotting, we demonstrated that PPARγ expression was decreased when L-glutamine was added to the medium. Using immunohistochemistry, we demonstrated that PPARγ immunostaining was mainly found in cytoplasm when cells were cultured with L-glutamine while it was found in nuclei and cytoplasm when cells were grown without the addition of L-glutamine. Supershift retardation assays demonstrated a decrease of PPARγ binding onto consensus peroxisome proliferator response element. We concluded that L-glutamine modulated PPARγ expression in Caco-2 cells.

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December 2008
Volume 22 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Fiatte C, Huin C, Collet P, Plénat F, Dauça M and Schohn H: Expression of PPARγ is reduced by medium supplementation with L-glutamine in human colorectal Caco-2 cells. Int J Mol Med 22: 825-832, 2008
APA
Fiatte, C., Huin, C., Collet, P., Plénat, F., Dauça, M., & Schohn, H. (2008). Expression of PPARγ is reduced by medium supplementation with L-glutamine in human colorectal Caco-2 cells. International Journal of Molecular Medicine, 22, 825-832. https://doi.org/10.3892/ijmm_00000091
MLA
Fiatte, C., Huin, C., Collet, P., Plénat, F., Dauça, M., Schohn, H."Expression of PPARγ is reduced by medium supplementation with L-glutamine in human colorectal Caco-2 cells". International Journal of Molecular Medicine 22.6 (2008): 825-832.
Chicago
Fiatte, C., Huin, C., Collet, P., Plénat, F., Dauça, M., Schohn, H."Expression of PPARγ is reduced by medium supplementation with L-glutamine in human colorectal Caco-2 cells". International Journal of Molecular Medicine 22, no. 6 (2008): 825-832. https://doi.org/10.3892/ijmm_00000091