Danazol enhances Fas-mediated apoptosis in human endometrial epithelial cells within normal physiology

  • Authors:
    • Tetsuji Tanaka
    • Naohiko Umesaki
  • View Affiliations

  • Published online on: February 1, 2009     https://doi.org/10.3892/ijmm_00000122
  • Pages: 237-243
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Abstract

Local danazol therapy reduces the signs and symptoms of endometriosis without inhibition of regular ovulation and menstruation and without atrophic changes to the endometrium or vaginal wall. It has been suggested that danazol has possible non-cytotoxic direct actions on eutopic endometrial cells and endometriotic cells. We have investigated the direct effects of danazol on a human endometrial epithelial cell line, HHUA, which is believed to retain many normal intracellular signaling pathways. A thoroughly dissolved solution of danazol enhanced Fas-mediated apoptosis in HHUA cells without inhibiting cell proliferation. Semi-quantitative flow cytometric analysis revealed that danazol did not enhance cell surface expression of Fas antigens. The enhancement of Fas-mediated apoptosis by endometrial cytokines such as EGF, IL-1β and interferon-γ was not additively enhanced by danazol; nor did danazol enhance growth suppression by anticancer drugs such as paclitaxel, carboplatin and 5-fluorouracil. Moreover, danazol did not enhance the irradiation-induced cell growth suppression of radiation-sensitive human cervical squamous cancer ME180 cells. These results indicate that danazol may regulate endometrial epithelial cell proliferation and apoptosis within normal physiology.

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February 2009
Volume 23 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Tanaka T and Tanaka T: Danazol enhances Fas-mediated apoptosis in human endometrial epithelial cells within normal physiology. Int J Mol Med 23: 237-243, 2009
APA
Tanaka, T., & Tanaka, T. (2009). Danazol enhances Fas-mediated apoptosis in human endometrial epithelial cells within normal physiology. International Journal of Molecular Medicine, 23, 237-243. https://doi.org/10.3892/ijmm_00000122
MLA
Tanaka, T., Umesaki, N."Danazol enhances Fas-mediated apoptosis in human endometrial epithelial cells within normal physiology". International Journal of Molecular Medicine 23.2 (2009): 237-243.
Chicago
Tanaka, T., Umesaki, N."Danazol enhances Fas-mediated apoptosis in human endometrial epithelial cells within normal physiology". International Journal of Molecular Medicine 23, no. 2 (2009): 237-243. https://doi.org/10.3892/ijmm_00000122