Epigallocatechin-3-gallate improves nonalcoholic steatohepatitis model mice expressing nuclear sterol regulatory element binding protein-1c in adipose tissue

  • Authors:
    • Takato Ueno
    • Takuji Torimura
    • Toru Nakamura
    • Ramadoss Sivakumar
    • Hitomi Nakayama
    • Syuichi Otabe
    • Xiaohong Yuan
    • Kentaro Yamada
    • Osamu Hashimoto
    • Kinya Inoue
    • Hironori Koga
    • Michio Sata
  • View Affiliations

  • Published online on: July 1, 2009     https://doi.org/10.3892/ijmm_00000200
  • Pages: 17-22
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Abstract

We examined whether or not epigallocatechin-3-gallate (EGCG) improves liver injury of nonalcoholic steatohepatitis (NASH) model mice expressing nuclear sterol regulatory element-binding protein 1c (nSREBP-1c) in adipose tissue. nSREBP-1c transgenic C57BL6 mice aged 30 weeks were divided into group 1 (no treatment), group 2 (ascorbic acid alone), group 3 (ascorbic acid and 0.05% EGCG), and group 4 (ascorbic acid and 0.1% EGCG). At 42 weeks, we performed measurement of liver weight to body weight, biochemical assays, morphometry of liver specimens, immunohistochemistry for 8-hydro-2'-deoxyguanosine (8-OhdG), and Western blotting for insulin and TNF-α signalings. Ratio of liver weight to body weight in the high dose EGCG-treated group (group 4) was significantly lower than those of groups 1 and 2 (p<0.05 and <0.01, respectively). Blood ALT, glucose, total cholesterol, and triglyceride levels of group 4 were significantly low compared with those of the EGCG-non-treated group (groups 1 and 2) (p<0.05, respectively). The degrees of steatosis, inflammation, ballooning hepatocytes and Mallory-Denk bodies in group 4 significantly improved compared with those in other groups (p<0.05, respectively). The 8-OhdG immunolocalization in liver tissues of the group 4 obviously decreased compared with those of groups 2 and 3. For Western blotting, the expressions of insulin receptor substrate-1 (IRS-1) and phosphorylated IRS-1 (pIRS-1) in liver tissues of group 4 increased compared with those of groups 2 and 3. On the other hand, the expressions of pAkt, pIKKβ and pNF-κB decreased compared with those of groups 2 and 3. From these results, EGCG reduces inflammation, insulin resistance and oxidative stress, and suppresses liver injury in nSREBP-1c transgenic mice.

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July 2009
Volume 24 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Ueno T, Torimura T, Nakamura T, Sivakumar R, Nakayama H, Otabe S, Yuan X, Yamada K, Hashimoto O, Inoue K, Inoue K, et al: Epigallocatechin-3-gallate improves nonalcoholic steatohepatitis model mice expressing nuclear sterol regulatory element binding protein-1c in adipose tissue. Int J Mol Med 24: 17-22, 2009
APA
Ueno, T., Torimura, T., Nakamura, T., Sivakumar, R., Nakayama, H., Otabe, S. ... Sata, M. (2009). Epigallocatechin-3-gallate improves nonalcoholic steatohepatitis model mice expressing nuclear sterol regulatory element binding protein-1c in adipose tissue. International Journal of Molecular Medicine, 24, 17-22. https://doi.org/10.3892/ijmm_00000200
MLA
Ueno, T., Torimura, T., Nakamura, T., Sivakumar, R., Nakayama, H., Otabe, S., Yuan, X., Yamada, K., Hashimoto, O., Inoue, K., Koga, H., Sata, M."Epigallocatechin-3-gallate improves nonalcoholic steatohepatitis model mice expressing nuclear sterol regulatory element binding protein-1c in adipose tissue". International Journal of Molecular Medicine 24.1 (2009): 17-22.
Chicago
Ueno, T., Torimura, T., Nakamura, T., Sivakumar, R., Nakayama, H., Otabe, S., Yuan, X., Yamada, K., Hashimoto, O., Inoue, K., Koga, H., Sata, M."Epigallocatechin-3-gallate improves nonalcoholic steatohepatitis model mice expressing nuclear sterol regulatory element binding protein-1c in adipose tissue". International Journal of Molecular Medicine 24, no. 1 (2009): 17-22. https://doi.org/10.3892/ijmm_00000200