In vitro and in vivo antimicrobial activity of water-soluble chitosan oligosaccharides against Vibrio vulnificus

  • Authors:
    • Byung Cheol Lee
    • Myun Soo Kim
    • Sang Ho Choi
    • Kwang Yoon Kim
    • Tae Sung Kim
  • View Affiliations

  • Published online on: September 1, 2009     https://doi.org/10.3892/ijmm_00000236
  • Pages: 327-333
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Abstract

Vibrio vulnificus is a Gram-negative bacterium that induces severely rapid pathological progress. In this study, we evaluated the antibacterial activity of two water-soluble chitosan oligosaccharides, COS A (MW, 10,000 Da) and COS B (MW, 1,000 Da), from 90-95% deacetylated chitosan, against V. vulnificus in vitro and in vivo. Treatment with COS A resulted in significantly higher suppressive effects on the growth of V. vulnificus than treatment with COS B. The growth of V. vulnificus was inhibited within 1 h of treatment with water-soluble COS A in concentrations ranging from 0.5 to 10 mg/ml. Additionally, treatment with COS A completely inhibited V. vulnificus-induced cytotoxicity in human intestinal epithelial INT-407 cells, while COS B did not. Furthermore, the administration of COS A (0.1-0.5 mg per mouse) significantly increased the survival period of V. vulnificus-infected mice. The number of viable V. vulnificus in the spleen, liver, small intestine, and blood was significantly lower in COS A-treated mice than in untreated mice. Here, we clearly demonstrate that COS A is a potential agent for the prevention and treatment of infection with V. vulnificus.

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September 2009
Volume 24 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Lee BC, Kim MS, Choi SH, Kim KY and Kim TS: In vitro and in vivo antimicrobial activity of water-soluble chitosan oligosaccharides against Vibrio vulnificus. Int J Mol Med 24: 327-333, 2009
APA
Lee, B.C., Kim, M.S., Choi, S.H., Kim, K.Y., & Kim, T.S. (2009). In vitro and in vivo antimicrobial activity of water-soluble chitosan oligosaccharides against Vibrio vulnificus. International Journal of Molecular Medicine, 24, 327-333. https://doi.org/10.3892/ijmm_00000236
MLA
Lee, B. C., Kim, M. S., Choi, S. H., Kim, K. Y., Kim, T. S."In vitro and in vivo antimicrobial activity of water-soluble chitosan oligosaccharides against Vibrio vulnificus". International Journal of Molecular Medicine 24.3 (2009): 327-333.
Chicago
Lee, B. C., Kim, M. S., Choi, S. H., Kim, K. Y., Kim, T. S."In vitro and in vivo antimicrobial activity of water-soluble chitosan oligosaccharides against Vibrio vulnificus". International Journal of Molecular Medicine 24, no. 3 (2009): 327-333. https://doi.org/10.3892/ijmm_00000236