λ-Carrageenan oligosaccharides elicit reactive oxygen species production resulting in mitochondrial-dependent apoptosis in human umbilical vein endothelial cells

  • Authors:
    • Hai-Min Chen
    • Xiao-Jun Yan
    • Ting-Yan Mai
    • Feng Wang
    • Wei-Feng Xu
  • View Affiliations

  • Published online on: December 1, 2009     https://doi.org/10.3892/ijmm_00000295
  • Pages: 801-806
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Abstract

Previous studies have shown that highly sulfated λ-carrageenan oligosaccharides (λ-CO) possess an anti-angiogenetic effect, while high concentrations of λ-CO present a cytotoxic effect towards human umbilical vein endothelial cells (HUVECs). The aim of this study was to explore the underlying mechanism of λ-CO on inhibiting cell proliferation. λ-CO elicited reactive oxygen species (ROS) production with concentrations at 0.8 and 1 mg/ml, and this event was accompanied by the increase of early apoptotic cells, nuclear morphology changes and cell cycle arrest at the S and G2/M phases. However, prevention of oxidative stress by N-acetyl-L-cysteine (NAC) could abolish the effect of λ-CO on these events. Yet, λ-CO induced a depolarization of mitochondrial transmembrane potential. At 0.8 mg/ml, λ-CO induced up-regulation of p53 and Bax, down-regulation of Bcl-2 and activation of caspase-9 and -3. These results suggest that exposure to a high concentration of λ-CO activates the mitochondrial-mediated apoptotic pathway and cell cycle arrest by generation of ROS.

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December 2009
Volume 24 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Chen H, Yan X, Mai T, Wang F and Xu W: λ-Carrageenan oligosaccharides elicit reactive oxygen species production resulting in mitochondrial-dependent apoptosis in human umbilical vein endothelial cells . Int J Mol Med 24: 801-806, 2009
APA
Chen, H., Yan, X., Mai, T., Wang, F., & Xu, W. (2009). λ-Carrageenan oligosaccharides elicit reactive oxygen species production resulting in mitochondrial-dependent apoptosis in human umbilical vein endothelial cells . International Journal of Molecular Medicine, 24, 801-806. https://doi.org/10.3892/ijmm_00000295
MLA
Chen, H., Yan, X., Mai, T., Wang, F., Xu, W."λ-Carrageenan oligosaccharides elicit reactive oxygen species production resulting in mitochondrial-dependent apoptosis in human umbilical vein endothelial cells ". International Journal of Molecular Medicine 24.6 (2009): 801-806.
Chicago
Chen, H., Yan, X., Mai, T., Wang, F., Xu, W."λ-Carrageenan oligosaccharides elicit reactive oxygen species production resulting in mitochondrial-dependent apoptosis in human umbilical vein endothelial cells ". International Journal of Molecular Medicine 24, no. 6 (2009): 801-806. https://doi.org/10.3892/ijmm_00000295