Open Access

Establishment and characterization of gastric carcinoma cell clones expressing LMP2A of Epstein-Barr virus

  • Authors:
    • Jung Seon Seo
    • Se Mo Jun
    • Sang Wook Kwon
    • Il-Hoan Oh
    • Tai-Gyu Kim
    • Suk Kyeong Lee
  • View Affiliations

  • Published online on: January 1, 2010     https://doi.org/10.3892/ijmm_00000307
  • Pages: 11-16
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Abstract

Although Epstein-Barr virus (EBV) has been detected in 5-15% of gastric carcinoma (GC) cases, the mechanism by which EBV contributes to its tumorigenesis remains unclear. Only a subset of EBV latent proteins such as EBV nuclear antigen-1 and latent membrane protein 2A (LMP2A) are expressed in EBV-associated GC (EBVaGC) cases. In this study, to elucidate the role of LMP2A in the tumorigenesis of EBVaGC, we established permanent cell lines expressing LMP2A. The LMP2A gene was cloned from a naturally EBV-infected EBVaGC cell line, SNU-719 and transduced into an EBV-negative GC cell line, AGS, using a retroviral vector. The sequence of SNU-719 LMP2A showed several conserved variations compared to that of the prototype EBV strain B95-8 LMP2A. Four of seven established cell clones expressed LMP2A protein at detectable levels. These cell clones did not show enhanced cell growth compared to control cells in normal or low serum-containing medium. Furthermore, LMP2A expression had no effect on colony forming ability of the cell clones in soft agar. Our results suggest that LMP2A alone has little effect on tumorigenesis of EBVaGC.

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Journal Cover

January 2010
Volume 25 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Seo JS, Jun SM, Kwon SW, Oh I, Kim T and Lee SK: Establishment and characterization of gastric carcinoma cell clones expressing LMP2A of Epstein-Barr virus. Int J Mol Med 25: 11-16, 2010
APA
Seo, J.S., Jun, S.M., Kwon, S.W., Oh, I., Kim, T., & Lee, S.K. (2010). Establishment and characterization of gastric carcinoma cell clones expressing LMP2A of Epstein-Barr virus. International Journal of Molecular Medicine, 25, 11-16. https://doi.org/10.3892/ijmm_00000307
MLA
Seo, J. S., Jun, S. M., Kwon, S. W., Oh, I., Kim, T., Lee, S. K."Establishment and characterization of gastric carcinoma cell clones expressing LMP2A of Epstein-Barr virus". International Journal of Molecular Medicine 25.1 (2010): 11-16.
Chicago
Seo, J. S., Jun, S. M., Kwon, S. W., Oh, I., Kim, T., Lee, S. K."Establishment and characterization of gastric carcinoma cell clones expressing LMP2A of Epstein-Barr virus". International Journal of Molecular Medicine 25, no. 1 (2010): 11-16. https://doi.org/10.3892/ijmm_00000307