Millimeter wave treatment inhibits NO-induced apoptosis of chondrocytes through the p38MAPK pathway

  • Authors:
    • Xihai Li
    • Min Du
    • Xianxiang Liu
    • Mingxia Wu
    • Hongzhi Ye
    • Jiumao Lin
    • Wenlie Chen
    • Guangwen Wu
  • View Affiliations

  • Published online on: March 1, 2010     https://doi.org/10.3892/ijmm_00000357
  • Pages: 393-399
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Abstract

In the present study, we investigated the effects of millimeter wave treatment on the activation of the p38MAPK signaling pathway in the process of NO-induced apoptosis in chondrocytes. Cartilage was isolated from the knee joint of SD rats and used to establish cultured primary chondrocytes. After identification using in situ staining of type II collagen, the passage 2 chondrocytes were incubated with or without sodium nitroprussiate (SNP) to induce apoptosis and treated with a millimeter wave for various times. The apoptosis of chondrocytes was detected using immunofluorescence, an MTT assay, and Annexin V-FITC labeling followed by fluorescence-activated cell sorting (FACS). The activity of caspase-3 was measured using colorimeters, and the levels of p38 and p53 were also detected using RT-PCR and Western blotting. After treatment with SNP, the OD values of the experimental groups were significantly lower than the control group (P<0.01). The 24-h interference of a millimeter wave significantly prevented apoptosis (P<0.01) and showed a dose dependency, and an identical trend of apoptosis was noted with normal cell number counting (P<0.01) and FACS (P<0.01). Consistently, the caspase 3 activity showed a reverse trend, with the highest activity in the experimental group receiving no millimeter wave treatment (P<0.01). The mRNA expression of p38 and p53 and the protein levels of phosphorylated p38 and p53 showed a similar trend (P<0.01) to that of caspase 3 activity. In conclusion, millimeter wave treatment inhibits the SNP-induced apoptosis of chondrocytes through the p38MAPK pathway.

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March 2010
Volume 25 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Li X, Du M, Liu X, Wu M, Ye H, Lin J, Chen W and Wu G: Millimeter wave treatment inhibits NO-induced apoptosis of chondrocytes through the p38MAPK pathway. Int J Mol Med 25: 393-399, 2010
APA
Li, X., Du, M., Liu, X., Wu, M., Ye, H., Lin, J. ... Wu, G. (2010). Millimeter wave treatment inhibits NO-induced apoptosis of chondrocytes through the p38MAPK pathway. International Journal of Molecular Medicine, 25, 393-399. https://doi.org/10.3892/ijmm_00000357
MLA
Li, X., Du, M., Liu, X., Wu, M., Ye, H., Lin, J., Chen, W., Wu, G."Millimeter wave treatment inhibits NO-induced apoptosis of chondrocytes through the p38MAPK pathway". International Journal of Molecular Medicine 25.3 (2010): 393-399.
Chicago
Li, X., Du, M., Liu, X., Wu, M., Ye, H., Lin, J., Chen, W., Wu, G."Millimeter wave treatment inhibits NO-induced apoptosis of chondrocytes through the p38MAPK pathway". International Journal of Molecular Medicine 25, no. 3 (2010): 393-399. https://doi.org/10.3892/ijmm_00000357