Solvent-induced virus inactivation by acidic arginine solution

  • Authors:
    • Kazuko Tsujimoto
    • Misao Uozaki
    • Keiko Ikeda
    • Hisashi Yamazaki
    • Hirotoshi Utsunomiya
    • Masao Ichinose
    • A. Hajime Koyama
    • Tsutomu Arakawa
  • View Affiliations

  • Published online on: March 1, 2010     https://doi.org/10.3892/ijmm_00000362
  • Pages: 433-437
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Abstract

Viral clearance is a primary concern for parenteral protein biopharmaceuticals. Low pH, detergent/solvent wash, or heating, called pasteurization, has been the main process for virus inactivation. Detergent/solvent wash is also used to treat superficial infectious diseases, including herpes simplex virus (HSV) infections. Herein we examined virus inactivation effects of acidic arginine on HSV type 2 (HSV-2) as a function of pH and temperature in an attempt to find solvent conditions that are effective for virus inactivation, yet are compatible with in vivo applications. Aqueous arginine at 0.7 M was highly effective on HSV-2, more so at lower pH and higher temperature. Its effects were stronger than 0.1 M citrate, 0.1 M citrate/0.6 M NaCl or 0.7 M citrate at any pH and temperature. This demonstrates that strong virus inactivation effects of arginine are not simply due to ionic strength or high concentration and arginine possesses a unique property that results in irreversible damage in virus particles. Such strong virus inactivation effects can be used in vivo for certain superficial infectious diseases, such as genital infections.

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March 2010
Volume 25 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Tsujimoto K, Uozaki M, Ikeda K, Yamazaki H, Utsunomiya H, Ichinose M, Koyama AH and Arakawa T: Solvent-induced virus inactivation by acidic arginine solution. Int J Mol Med 25: 433-437, 2010
APA
Tsujimoto, K., Uozaki, M., Ikeda, K., Yamazaki, H., Utsunomiya, H., Ichinose, M. ... Arakawa, T. (2010). Solvent-induced virus inactivation by acidic arginine solution. International Journal of Molecular Medicine, 25, 433-437. https://doi.org/10.3892/ijmm_00000362
MLA
Tsujimoto, K., Uozaki, M., Ikeda, K., Yamazaki, H., Utsunomiya, H., Ichinose, M., Koyama, A. H., Arakawa, T."Solvent-induced virus inactivation by acidic arginine solution". International Journal of Molecular Medicine 25.3 (2010): 433-437.
Chicago
Tsujimoto, K., Uozaki, M., Ikeda, K., Yamazaki, H., Utsunomiya, H., Ichinose, M., Koyama, A. H., Arakawa, T."Solvent-induced virus inactivation by acidic arginine solution". International Journal of Molecular Medicine 25, no. 3 (2010): 433-437. https://doi.org/10.3892/ijmm_00000362