Alteration of T helper cell subsets in the optic nerve of experimental autoimmune encephalomyelitis

  • Authors:
    • A-Yong Tian
    • Rong-Wei Zhang
    • Xiao-Guang Shi
    • Hong-Mei Yu
  • View Affiliations

  • Published online on: June 1, 2010     https://doi.org/10.3892/ijmm_00000416
  • Pages: 869-874
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Abstract

The objective of this study was to detect interleukin-17 (IL-17), interferon-γ (IFN-γ), interleukin-4 (IL-4) and forkhead/winged helix transcription factor p3 (Foxp3) protein and gene expression of the optic nerve and to further explore the role of T helper cell subsets such as Th1, Th2, Th17 and Treg in the pathogenesis of optic neuritis in experimental autoimmune encephalomyelitis (EAE). Mice in C57BL/6 background were randomly divided into control and EAE groups. At days 11, 15 and 19 post-immunization, optic nerves were dissected for morphological study to detect IL-17, IFN-γ and IL-4. Protein analysis was done by enzyme-linked immunosorbent assay, quantitative real-time polymerase chain reaction for measuring the gene expression of IL-17, IFN-γ, IL-4 and Foxp3. Concentrations of IL-17 protein in the optic nerve were significantly up-regulated at 11 days post-immunization, and IFN-γ protein concentrations at 19 days. Concentrations of IL-4 protein in the optic nerve declined slightly in 19 days. mRNA expression of IL-17, IFN-γ and IL-4 was consistent with their protein expression. Foxp3 mRNA transcription was down-regulated at 11-19 days post-immunization. Decreased expression of Foxp3 mRNA and Treg in the optic nerve may play a key role in the development of optic neuritis. IL-17 may mediate inflammatory pathogenicity at the early stage of optic neuritis, and IFN-γ may aggravate inflammatory injury during the peak stage of optic neuritis.

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June 2010
Volume 25 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Tian A, Zhang R, Shi X and Yu H: Alteration of T helper cell subsets in the optic nerve of experimental autoimmune encephalomyelitis . Int J Mol Med 25: 869-874, 2010
APA
Tian, A., Zhang, R., Shi, X., & Yu, H. (2010). Alteration of T helper cell subsets in the optic nerve of experimental autoimmune encephalomyelitis . International Journal of Molecular Medicine, 25, 869-874. https://doi.org/10.3892/ijmm_00000416
MLA
Tian, A., Zhang, R., Shi, X., Yu, H."Alteration of T helper cell subsets in the optic nerve of experimental autoimmune encephalomyelitis ". International Journal of Molecular Medicine 25.6 (2010): 869-874.
Chicago
Tian, A., Zhang, R., Shi, X., Yu, H."Alteration of T helper cell subsets in the optic nerve of experimental autoimmune encephalomyelitis ". International Journal of Molecular Medicine 25, no. 6 (2010): 869-874. https://doi.org/10.3892/ijmm_00000416