Increased expression of calpain and elevated activity of calcineurin in the myocardium of patients with congestive heart failure

  • Authors:
    • Dachun Yang
    • Shuangtao Ma
    • Yan Tan
    • De Li
    • Bing Tang
    • Xin Zhang
    • Meiqin Sun
    • Yongjian Yang
  • View Affiliations

  • Published online on: July 1, 2010     https://doi.org/10.3892/ijmm_00000448
  • Pages: 159-164
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Abstract

The angiotensin (Ang) II/Ang II receptor (ATR)- associated calcium signaling pathway is the major cause of ventricular remodelling in patients with congestive heart failure (CHF). However, the calcium-regulated proteinases responsible for Ang II-induced remodelling are not well understood. We investigated the profiles of the Ang II/ATR/calpain/calcineurin (CaN) pathway in human failing heart. We measured both the plasma and cardiac levels of Ang II and cardiac mRNA expression of ATR in 39 patients with CHF and 38 healthy controls. Importantly, protein expression of calpains, cleavage of cain/cabin1 and activity of CaN were tested. Both plasma and cardiac levels of Ang II were significantly increased in patients with CHF (both p<0.01), and the plasma Ang II concentration was closely correlated with the parameters of ventricular remodelling (r=±0.29-0.65, p<0.05 or <0.01). In addition, the cardiac level of AT1R but not AT2R was significantly upregulated in mild failing hearts (p<0.05) but dramatically downregulated in severe failing ones (p<0.01). CHF was associated with a marked upregulation of calpains, an increased cleavage of cain/cabin1, and the activation of CaN in the failing ventricular tissue. In patients with CHF, calpain upregulation was associated with an increase in cleavage of cain/cabin1 and the activation of CaN, indicating that these changes in calcium-regulated proteinases contribute to Ang II-induced cardiac remodelling.

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July 2010
Volume 26 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Yang D, Ma S, Tan Y, Li D, Tang B, Zhang X, Sun M and Yang Y: Increased expression of calpain and elevated activity of calcineurin in the myocardium of patients with congestive heart failure . Int J Mol Med 26: 159-164, 2010
APA
Yang, D., Ma, S., Tan, Y., Li, D., Tang, B., Zhang, X. ... Yang, Y. (2010). Increased expression of calpain and elevated activity of calcineurin in the myocardium of patients with congestive heart failure . International Journal of Molecular Medicine, 26, 159-164. https://doi.org/10.3892/ijmm_00000448
MLA
Yang, D., Ma, S., Tan, Y., Li, D., Tang, B., Zhang, X., Sun, M., Yang, Y."Increased expression of calpain and elevated activity of calcineurin in the myocardium of patients with congestive heart failure ". International Journal of Molecular Medicine 26.1 (2010): 159-164.
Chicago
Yang, D., Ma, S., Tan, Y., Li, D., Tang, B., Zhang, X., Sun, M., Yang, Y."Increased expression of calpain and elevated activity of calcineurin in the myocardium of patients with congestive heart failure ". International Journal of Molecular Medicine 26, no. 1 (2010): 159-164. https://doi.org/10.3892/ijmm_00000448