Human RCAN3 gene expression and cell growth in endothelial cells

  • Authors:
    • Silvia Canaider
    • Marina Vettraino
    • Lucy V. Norling
    • Enzo Spisni
    • Federica Facchin
    • Dianne Cooper
    • Mauro Perretti
  • View Affiliations

  • Published online on: December 1, 2010     https://doi.org/10.3892/ijmm_00000542
  • Pages: 913-918
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Regulator of calcineurin 3 (RCAN3) belongs to the human RCAN gene family, which also includes RCAN1 and RCAN2. All three members interact with and inhibit calcineurin. Based on this effect, several studies have demonstrated a role for RCAN1 and RCAN2 on inflammation, using human umbilical vein endothelial cells (HUVECs) as a model. RCAN1 and 2 are strongly induced by vascular endothelial growth factor (VEGF), inhibit cell proliferation and down-regulate many pro-inflammatory and pro-angiogenic genes. The present work is the first study to investigate the role of RCAN3 on inflammation in HUVECs. RCAN3 isoforms have been characterized and quantified in HUVECs; only those with the same frame are expressed and show a peculiar expression pattern. RCAN3 inhibits HUVEC proliferation both basally and under VEGF or phorbol 12-myristate 13-acetate-stimulated conditions, however it does not modulate gene expression of the chosen inflammatory genes. Results indicate an interesting role for RCAN3 in modulating HUVEC proliferation, independently from the inflammatory and angiogenic processes.

Related Articles

Journal Cover

December 2010
Volume 26 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Canaider S, Vettraino M, Norling LV, Spisni E, Facchin F, Cooper D and Perretti M: Human RCAN3 gene expression and cell growth in endothelial cells . Int J Mol Med 26: 913-918, 2010
APA
Canaider, S., Vettraino, M., Norling, L.V., Spisni, E., Facchin, F., Cooper, D., & Perretti, M. (2010). Human RCAN3 gene expression and cell growth in endothelial cells . International Journal of Molecular Medicine, 26, 913-918. https://doi.org/10.3892/ijmm_00000542
MLA
Canaider, S., Vettraino, M., Norling, L. V., Spisni, E., Facchin, F., Cooper, D., Perretti, M."Human RCAN3 gene expression and cell growth in endothelial cells ". International Journal of Molecular Medicine 26.6 (2010): 913-918.
Chicago
Canaider, S., Vettraino, M., Norling, L. V., Spisni, E., Facchin, F., Cooper, D., Perretti, M."Human RCAN3 gene expression and cell growth in endothelial cells ". International Journal of Molecular Medicine 26, no. 6 (2010): 913-918. https://doi.org/10.3892/ijmm_00000542