Chamomile: An anti-inflammatory agent inhibits inducible nitric oxide synthase expression by blocking RelA/p65 activity

  • Authors:
    • Natarajan Bhaskaran
    • Sanjeev Shukla
    • Janmejai K. Srivastava
    • Sanjay Gupta
  • View Affiliations

  • Published online on: December 1, 2010     https://doi.org/10.3892/ijmm_00000545
  • Pages: 935-940
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Abstract

Chamomile has long been used in traditional medicine for the treatment of inflammation-related disorders. In this study we investigated the inhibitory effects of chamomile on nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) expression, and explored its potential anti-inflammatory mechanisms using RAW 264.7 macrophages. Chamomile treatment inhibited LPS-induced NO production and significantly blocked IL-1β, IL-6 and TNFα-induced NO levels in RAW 264.7 macrophages. Chamomile caused reduction in LPS-induced iNOS mRNA and protein expression. In RAW 264.7 macrophages, LPS-induced DNA binding activity of RelA/p65 was significantly inhibited by chamomile, an effect that was mediated through the inhibition of IKKβ, the upstream kinase regulating NF-κB/Rel activity, and degradation of inhibitory factor-κB. These results demonstrate that chamomile inhibits NO production and iNOS gene expression by inhibiting RelA/p65 activation and supports the utilization of chamomile as an effective anti-inflammatory agent.

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December 2010
Volume 26 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Bhaskaran N, Shukla S, Srivastava JK and Gupta S: Chamomile: An anti-inflammatory agent inhibits inducible nitric oxide synthase expression by blocking RelA/p65 activity . Int J Mol Med 26: 935-940, 2010
APA
Bhaskaran, N., Shukla, S., Srivastava, J.K., & Gupta, S. (2010). Chamomile: An anti-inflammatory agent inhibits inducible nitric oxide synthase expression by blocking RelA/p65 activity . International Journal of Molecular Medicine, 26, 935-940. https://doi.org/10.3892/ijmm_00000545
MLA
Bhaskaran, N., Shukla, S., Srivastava, J. K., Gupta, S."Chamomile: An anti-inflammatory agent inhibits inducible nitric oxide synthase expression by blocking RelA/p65 activity ". International Journal of Molecular Medicine 26.6 (2010): 935-940.
Chicago
Bhaskaran, N., Shukla, S., Srivastava, J. K., Gupta, S."Chamomile: An anti-inflammatory agent inhibits inducible nitric oxide synthase expression by blocking RelA/p65 activity ". International Journal of Molecular Medicine 26, no. 6 (2010): 935-940. https://doi.org/10.3892/ijmm_00000545