The antagonist of the JAK-1/STAT-1 signaling pathway improves the severity of cerulein-stimulated pancreatic injury via inhibition of NF-κB activity

  • Authors:
    • Ping Chen
    • Liya Huang
    • Yongping Zhang
    • Minmin Qiao
    • Weiyan Yao
    • Yaozong Yuan
  • View Affiliations

  • Published online on: March 1, 2011     https://doi.org/10.3892/ijmm.2011.632
  • Pages: 731-738
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Abstract

The Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway is widely involved in cell migration, apoptosis and inflammation. However, its exact mechanisms in severe acute pancreatitis (SAP) remain unclear. The aim of this study was to explore the activity of the JAK/STAT signaling pathway in pancreatic injury, investigate the functional mechanisms of SAP in vitro, and thus elucidate the underlying therapeutic effects for SAP in vivo. The activation of the JAK-1/STAT-1 signaling pathway and the expessions of TNF-α, IL-1β and IL-6 proteins were investigated in AR42J cells induced with cerulein and treated with either PBS, RPM, or AG490. One group of cells was left untreated as a control group. Subsequently the activity of NF-κB was evaluated. Rats were given RPM or AG490 just before the induction of SAP, the severity of which was assessed at 24 h. The findings revealed that the up-regulated expressions of JAK-1/STAT-1, STAT-3 protein were closely correlated with the transcription of TNF-α, IL-1β, and IL-6 in cerulein-stimulated cells. Administration of RPM or AG490 decreased the activity of NF-κB and inhibited the release of TNF-α, IL-1β, and IL-6. The reflective markers of severity of SAP were also decreased by RPM or AG490 treatment compared to SAP rats. This study indicates that the JAK-1/STAT-1 signaling pathway activity is an early event in pancreatic inflammatory injury. Therefore, early treatment with its inhibitors might be beneficial for attenuation of pancreatic injury in SAP.

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May 2011
Volume 27 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Chen P, Huang L, Zhang Y, Qiao M, Yao W and Yuan Y: The antagonist of the JAK-1/STAT-1 signaling pathway improves the severity of cerulein-stimulated pancreatic injury via inhibition of NF-κB activity. Int J Mol Med 27: 731-738, 2011
APA
Chen, P., Huang, L., Zhang, Y., Qiao, M., Yao, W., & Yuan, Y. (2011). The antagonist of the JAK-1/STAT-1 signaling pathway improves the severity of cerulein-stimulated pancreatic injury via inhibition of NF-κB activity. International Journal of Molecular Medicine, 27, 731-738. https://doi.org/10.3892/ijmm.2011.632
MLA
Chen, P., Huang, L., Zhang, Y., Qiao, M., Yao, W., Yuan, Y."The antagonist of the JAK-1/STAT-1 signaling pathway improves the severity of cerulein-stimulated pancreatic injury via inhibition of NF-κB activity". International Journal of Molecular Medicine 27.5 (2011): 731-738.
Chicago
Chen, P., Huang, L., Zhang, Y., Qiao, M., Yao, W., Yuan, Y."The antagonist of the JAK-1/STAT-1 signaling pathway improves the severity of cerulein-stimulated pancreatic injury via inhibition of NF-κB activity". International Journal of Molecular Medicine 27, no. 5 (2011): 731-738. https://doi.org/10.3892/ijmm.2011.632