Enhancement of basic fibroblast growth factor-stimulated VEGF synthesis by Wnt3a in osteoblasts

  • Authors:
    • Haruhiko Tokuda
    • Seiji Adachi
    • Rie Matsushima-Nishiwaki
    • Kenji  Kato
    • Hideo Natsume
    • Takanobu Otsuka
    • Osamu Kozawa
  • View Affiliations

  • Published online on: March 8, 2011     https://doi.org/10.3892/ijmm.2011.644
  • Pages: 859-864
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Abstract

It is currently recognized that the Wnt signaling pathway regulates bone mass. We have previously reported that the basic fibroblast growth factor (FGF-2) stimulates the synthesis of the vascular endothelial growth factor (VEGF) at least in part via the p44/p42 mitogen-activated protein (MAP) kinase and the stress-activated protein kinase (SAPK)/c-Jun N-terminal kinase (JNK) in osteoblast-like MC3T3-E1 cells. In the present study, we investigated the effect of Wnt3a on FGF-2-stimulated VEGF synthesis in MC3T3-E1 cells. Wnt3a significantly augmented the FGF-2-stimulated VEGF release in a dose-dependent manner in the range between 1 and 30 ng/ml. Lithium chloride and SB216763, inhibitors of glycogen synthase kinase 3β (GSK3β), enhanced the FGF-2-stimulated VEGF release. Wnt3a did not affect the phosphorylation of the p44/p42 MAP kinase, SAPK/JNK, Akt, p38 MAP kinase or the p70 S6 kinase induced by FGF-2. Wnt3a and SB216763 increased the levels of VEGF mRNA expression induced by FGF-2. These results strongly suggest that Wnt3a enhances VEGF synthesis stimulated by FGF-2 via activation of the canonical Wnt/β-catenin pathway in osteoblasts.

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June 2011
Volume 27 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Tokuda H, Adachi S, Matsushima-Nishiwaki R, Kato K, Natsume H, Otsuka T and Kozawa O: Enhancement of basic fibroblast growth factor-stimulated VEGF synthesis by Wnt3a in osteoblasts. Int J Mol Med 27: 859-864, 2011
APA
Tokuda, H., Adachi, S., Matsushima-Nishiwaki, R., Kato, K., Natsume, H., Otsuka, T., & Kozawa, O. (2011). Enhancement of basic fibroblast growth factor-stimulated VEGF synthesis by Wnt3a in osteoblasts. International Journal of Molecular Medicine, 27, 859-864. https://doi.org/10.3892/ijmm.2011.644
MLA
Tokuda, H., Adachi, S., Matsushima-Nishiwaki, R., Kato, K., Natsume, H., Otsuka, T., Kozawa, O."Enhancement of basic fibroblast growth factor-stimulated VEGF synthesis by Wnt3a in osteoblasts". International Journal of Molecular Medicine 27.6 (2011): 859-864.
Chicago
Tokuda, H., Adachi, S., Matsushima-Nishiwaki, R., Kato, K., Natsume, H., Otsuka, T., Kozawa, O."Enhancement of basic fibroblast growth factor-stimulated VEGF synthesis by Wnt3a in osteoblasts". International Journal of Molecular Medicine 27, no. 6 (2011): 859-864. https://doi.org/10.3892/ijmm.2011.644