Open Access

Alteration of the proteome profile of the pancreas in diabetic rats induced by streptozotocin

  • Authors:
    • Yong-Liang Jiang
    • You Ning
    • Xiao-Lei Ma
    • Yan-Yan Liu
    • Yu Wang
    • Zeng Zhang
    • Chun-Xiao Shan
    • Yu-Dong Xu
    • Lei-Miao Yin
    • Yong-Qing Yang
  • View Affiliations

  • Published online on: May 9, 2011     https://doi.org/10.3892/ijmm.2011.696
  • Pages: 153-160
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Abstract

Type 1 diabetes mellitus (T1DM) is receiving increased attention. To obtain a better understanding of the mechanism underlying T1DM, we performed a proteomic study on a rat model induced by streptozotocin. Pancreatic proteins were separated by two-dimensional gel electrophoresis. Eighteen protein spots were differentially expressed (P<0.05) with 2-fold or more increased or decreased intensity in the diabetic rats as compared with controls, of which 11 protein spots were up-regulated and 7 protein spots were down-regulated. These protein spots were successfully identified by liquid chromatography-electrospray ionization tandem mass spectrometry. The 60 kDa heat shock protein, the carbonyl reductase 1 (Cbr1), the hydroxyacyl-CoA dehydrogenase, Δ3,5,Δ2,4-dienoyl-CoA isomerase, the elongation factor 1-δ, the 26S protease regulatory subunit 7 and the transitional endoplasmic reticulum ATPase were up-regulated, while the 78 kDa glucose-regulated protein, peroxiredoxin 4 and plakoglobin were down-regulated. The expression change of Cbr1 which is closely related to diabetic complications was further validated by western blotting. Our results and those of the bioinformatics analysis suggest that oxidative stress, the Wnt pathway, fatty acid degradation and glucose transport may be closely related to T1DM.

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August 2011
Volume 28 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Jiang Y, Ning Y, Ma X, Liu Y, Wang Y, Zhang Z, Shan C, Xu Y, Yin L, Yang Y, Yang Y, et al: Alteration of the proteome profile of the pancreas in diabetic rats induced by streptozotocin. Int J Mol Med 28: 153-160, 2011
APA
Jiang, Y., Ning, Y., Ma, X., Liu, Y., Wang, Y., Zhang, Z. ... Yang, Y. (2011). Alteration of the proteome profile of the pancreas in diabetic rats induced by streptozotocin. International Journal of Molecular Medicine, 28, 153-160. https://doi.org/10.3892/ijmm.2011.696
MLA
Jiang, Y., Ning, Y., Ma, X., Liu, Y., Wang, Y., Zhang, Z., Shan, C., Xu, Y., Yin, L., Yang, Y."Alteration of the proteome profile of the pancreas in diabetic rats induced by streptozotocin". International Journal of Molecular Medicine 28.2 (2011): 153-160.
Chicago
Jiang, Y., Ning, Y., Ma, X., Liu, Y., Wang, Y., Zhang, Z., Shan, C., Xu, Y., Yin, L., Yang, Y."Alteration of the proteome profile of the pancreas in diabetic rats induced by streptozotocin". International Journal of Molecular Medicine 28, no. 2 (2011): 153-160. https://doi.org/10.3892/ijmm.2011.696