Hsp40 regulates the amount of keratin proteins via ubiquitin-proteasome pathway in cultured human cells

  • Authors:
    • Shota Yamazaki
    • Asato Uchiumi
    • Yohtaro Katagata
  • View Affiliations

  • Published online on: November 7, 2011     https://doi.org/10.3892/ijmm.2011.826
  • Pages: 165-168
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Abstract

Keratins represent important structural components of intermediate filament proteins. Their expression profiles are remarkably tissue-specific. Recent data have shown that keratins associate with many proteins including heat shock proteins (HSP). We recently identified cell-specific keratin and HSP expression. We aimed to gain further insight into the regulation of keratins by specific inhibition through knockdown of Hsp40 in human keratinocyte cells. Keratin-HSP interaction in HaCaT cell lysate was evaluated by immunoprecipitation followed by Western blotting. Immunofluorescence, was used to examine the co-localization of keratins and Hsp40. Hsp40 depletion led to an increase in the levels of keratin proteins (K5, K14, K10) and a decrease in keratin ubiquitination without influencing keratin gene expression. Our results demonstrate direct or indirectly association of Hsp40 and imply that expressed keratin proteins were regulated by Hsp40 depending on the ubiquitin-proteasome pathway in HaCaT. Furthermore, the K10 differentiation marker was increased by knockdown of Hsp40. The results presented in this study indicate that Hsp40 is related to the differentiation exchange of keratin pairs.

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February 2012
Volume 29 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Yamazaki S, Uchiumi A and Katagata Y: Hsp40 regulates the amount of keratin proteins via ubiquitin-proteasome pathway in cultured human cells. Int J Mol Med 29: 165-168, 2012
APA
Yamazaki, S., Uchiumi, A., & Katagata, Y. (2012). Hsp40 regulates the amount of keratin proteins via ubiquitin-proteasome pathway in cultured human cells. International Journal of Molecular Medicine, 29, 165-168. https://doi.org/10.3892/ijmm.2011.826
MLA
Yamazaki, S., Uchiumi, A., Katagata, Y."Hsp40 regulates the amount of keratin proteins via ubiquitin-proteasome pathway in cultured human cells". International Journal of Molecular Medicine 29.2 (2012): 165-168.
Chicago
Yamazaki, S., Uchiumi, A., Katagata, Y."Hsp40 regulates the amount of keratin proteins via ubiquitin-proteasome pathway in cultured human cells". International Journal of Molecular Medicine 29, no. 2 (2012): 165-168. https://doi.org/10.3892/ijmm.2011.826