Molecular mapping of the regenerative niche in a murine model of myocardial infarction

  • Authors:
    • Gabriela Dornelles Alves
    • Mariana Pazzine
    • Luisa Maria Gomes de Macedo Braga
    • Maria Cláudia Irigoyen
    • Kátia De Angelis
    • Nilo Ikuta
    • Melissa Camassola
    • Nance Beyer Nardi
  • View Affiliations

  • Published online on: November 29, 2011     https://doi.org/10.3892/ijmm.2011.850
  • Pages: 479-484
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Abstract

Adult stem cells are distributed through the whole organism, and present a great potential for the therapy of different types of disease. For the design of efficient therapeutic strategies, it is important to have a more detailed understanding of their basic biological characteristics, as well as of the signals produced by damaged tissues and to which they respond. Myocardial infarction (MI), a disease caused by a lack of blood flow supply in the heart, represents the most common cause of morbidity and mortality in the Western world. Stem cell therapy arises as a promising alternative to conventional treatments, which are often ineffective in preventing loss of cardiomyocytes and fibrosis. Cell therapy protocols must take into account the molecular events that occur in the regenerative niche of MI. In the present study, we investigated the expression profile of ten genes coding for chemokines or cytokines in a murine model of MI, aiming at the characterization of the regenerative niche. MI was induced in adult C57BL/6 mice and heart samples were collected after 24 h and 30 days, as well as from control animals, for quantitative RT-PCR. Expression of the chemokine genes CCL2, CCL3, CCL4, CCL7, CXCL2 and CXCL10 was significantly increased 24 h after infarction, returning to baseline levels on day 30. Expression of the CCL8 gene significantly increased only on day 30, whereas gene expression of CXCL12 and CX3CL1 were not significantly increased in either ischemic period. Finally, expression of the IL-6 gene increased 24 h after infarction and was maintained at a significantly higher level than control samples 30 days later. These results contribute to the better knowledge of the regenerative niche in MI, allowing a more efficient selection or genetic manipulation of cells in therapeutic protocols.

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March 2012
Volume 29 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Alves GD, Pazzine M, Gomes de Macedo Braga LM, Irigoyen MC, De Angelis K, Ikuta N, Camassola M and Nardi NB: Molecular mapping of the regenerative niche in a murine model of myocardial infarction. Int J Mol Med 29: 479-484, 2012
APA
Alves, G.D., Pazzine, M., Gomes de Macedo Braga, L.M., Irigoyen, M.C., De Angelis, K., Ikuta, N. ... Nardi, N.B. (2012). Molecular mapping of the regenerative niche in a murine model of myocardial infarction. International Journal of Molecular Medicine, 29, 479-484. https://doi.org/10.3892/ijmm.2011.850
MLA
Alves, G. D., Pazzine, M., Gomes de Macedo Braga, L. M., Irigoyen, M. C., De Angelis, K., Ikuta, N., Camassola, M., Nardi, N. B."Molecular mapping of the regenerative niche in a murine model of myocardial infarction". International Journal of Molecular Medicine 29.3 (2012): 479-484.
Chicago
Alves, G. D., Pazzine, M., Gomes de Macedo Braga, L. M., Irigoyen, M. C., De Angelis, K., Ikuta, N., Camassola, M., Nardi, N. B."Molecular mapping of the regenerative niche in a murine model of myocardial infarction". International Journal of Molecular Medicine 29, no. 3 (2012): 479-484. https://doi.org/10.3892/ijmm.2011.850